Vector Labs Ab04571-3.0 Anti-CD9 [10E4], Mouse IgG2b, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 10E4
Synonyms: p24; MIC3; MRP-1; GIG2; Tspan-29; TSPAN29; 5H9 antigen; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; Motility-related protein; Tetraspanin-29
Amount: 100 μg
Application Codes Clone: FC; ELISA
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: scFv
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This full-length, chimeric mouse antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody specifically binds amino acids 'KEVFDN' in the extracellular loop 2 domain (CD9-ECL2) of human CD9. This protein is an intergral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion. Extracellular vesicles (EVs) such as exosomes are important mediators in intercellular communications and play a role in cancer progression and metastasis. Exosomal membranes are enriched in endosome-specific tetraspanins like CD9. EVs have strong potential as blood-based or urine-based biomarkers for the diagnosis, prognostication, and surveillance of cancer.
Application Notes: The binding of this antibody to CD9 expressed on different cancer cell lines like 2774 cells, U031 cells, ANBL-6 cells, 293E cells was tested using flow cytometry (US8197809). The epitope characterization of this antibody for fragment of CD9-ECL2 was measured using ELISA. This antibody significantly inhibited invasion of 2774 (ovarian adenocarcinoma cell line) cells and further inhibited migration and proliferation of 2774 cells (US8197809).

Product Class:	Purified
Clone ID:	10E4
Synonyms:	p24; MIC3; MRP-1; GIG2; Tspan-29; TSPAN29; 5H9 antigen; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; Motility-related protein; Tetraspanin-29
Amount:	100 μg
Application Codes Clone:	FC; ELISA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	scFv
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This full-length, chimeric mouse antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody specifically binds amino acids 'KEVFDN' in the extracellular loop 2 domain (CD9-ECL2) of human CD9. This protein is an intergral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion. Extracellular vesicles (EVs) such as exosomes are important mediators in intercellular communications and play a role in cancer progression and metastasis. Exosomal membranes are enriched in endosome-specific tetraspanins like CD9. EVs have strong potential as blood-based or urine-based biomarkers for the diagnosis, prognostication, and surveillance of cancer.
Application Notes:	The binding of this antibody to CD9 expressed on different cancer cell lines like 2774 cells, U031 cells, ANBL-6 cells, 293E cells was tested using flow cytometry (US8197809). The epitope characterization of this antibody for fragment of CD9-ECL2 was measured using ELISA. This antibody significantly inhibited invasion of 2774 (ovarian adenocarcinoma cell line) cells and further inhibited migration and proliferation of 2774 cells (US8197809).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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