Mfg Part Number
Ab04579-23.159
Vector Labs Ab04579-23.159 Anti-Capsid protein VP1 [Nano-25], Rabbit IgG-Fc Fusion, (100 μg)
Quick Overview
The original antibody was generated by immunizing a single alpaca with the GII.10 virus-like particle protein.
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| Product Class: | Purified |
|---|---|
| Clone ID: | Nano-25 |
| Synonyms: | CP; Capsid protein; ABCD_AG266 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; Crystallography; Cryo-EM |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 25520510 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Alpaca VHH format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the GII.10 group of the protruding (P) domain of the Norwalk virus capsid protein VP1. The P1 subdomain binding site is occluded on intact particles, yet this antibody binds intact norovirus VLPs and virions. |
| Application Notes: | The reactivity of the original antibody (alpaca VHH) against norovirus VLPs and P domains was determined using ELISA. Using isothermal titration calorimetry, a Kd of 28 nM was measured between the antibody and the GII.10 P domain. The crystal structure of this antibody in complex with norovirus GII.10 P domain was determined (Koromyslova & Hansman, 2015; PMID: 25520510). The epitope of this antibody was further characterized in X-ray crystallography and cryo-EM (Koromyslova & Hansman, 2017; PMID: 29095961). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |