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Mfg Part Number

Ab04580-34.11

Vector Labs Ab04580-34.11 Anti-Capsid protein VP1 [Nano-85], Camelid VHH, His-tagged, (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing a single alpaca with the GII.10 virus-like particle protein.

$692.90

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Description

Product Class: Purified
Clone ID: Nano-85
Heavy Chain Modification: His-tagged
Synonyms: CP; Capsid protein; ABCD_AG267; ABCD_AH279
Amount: 100 μg
Application Codes Clone: ELISA; Crystallography; Cryo-EM; Inhibition
Shipping Temperature: Wet Ice
Origin Pub PMID: 25520510
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: VHH
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody recognizes the protruding (P) domain of the Norwalk virus capsid protein VP1 with high affinity. Specifically, it binds the P1 subdomain (located in the lower region of the P domain), which is occluded on intact particles. Still, this antibody binds intact norovirus VLPs and virions. This antibody has been shown to bind to the GII.10, GII.1, GII.2, GII.4, GII.12, GII.17 and GI.11 genotypes.
Application Notes: The reactivity of the original antibody (alpaca VHH) against norovirus VLPs and P domains was determined using ELISA. It was shown to be reactive with antigenically distinct norovirus VLPs, including GII.4, GII.10, and GII.12. Using isothermal titration calorimetry, a K_d of 7 nM was measured against GII.10 P domain and a K_d of 3.5 nM against GII.4 P domain. The crystal structure of this antibody in complex with norovirus GII.10 P domain, Saga-2006 GII.4 P domain, and 2012 NSW GII.4 P domain were determined. This antibody can bind intact norovirus VLPs and virions and promotes their disassembly (Koromyslova & Hansman, 2015; PMID: 25520510). The epitope of this antibody was further characterized in X-ray crystallography and cryo-EM. This antibody's binding mechanism involves impairing capsid motions and integrity, which inhibits multiple stages of the Norovirus life cycle (Koromyslova & Hansman, 2017; PMID: 29095961).

Product Class:	Purified
Clone ID:	Nano-85
Heavy Chain Modification:	His-tagged
Synonyms:	CP; Capsid protein; ABCD_AG267; ABCD_AH279
Amount:	100 μg
Application Codes Clone:	ELISA; Crystallography; Cryo-EM; Inhibition
Shipping Temperature:	Wet Ice
Origin Pub PMID:	25520510
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	VHH
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody recognizes the protruding (P) domain of the Norwalk virus capsid protein VP1 with high affinity. Specifically, it binds the P1 subdomain (located in the lower region of the P domain), which is occluded on intact particles. Still, this antibody binds intact norovirus VLPs and virions. This antibody has been shown to bind to the GII.10, GII.1, GII.2, GII.4, GII.12, GII.17 and GI.11 genotypes.
Application Notes:	The reactivity of the original antibody (alpaca VHH) against norovirus VLPs and P domains was determined using ELISA. It was shown to be reactive with antigenically distinct norovirus VLPs, including GII.4, GII.10, and GII.12. Using isothermal titration calorimetry, a K_d of 7 nM was measured against GII.10 P domain and a K_d of 3.5 nM against GII.4 P domain. The crystal structure of this antibody in complex with norovirus GII.10 P domain, Saga-2006 GII.4 P domain, and 2012 NSW GII.4 P domain were determined. This antibody can bind intact norovirus VLPs and virions and promotes their disassembly (Koromyslova & Hansman, 2015; PMID: 25520510). The epitope of this antibody was further characterized in X-ray crystallography and cryo-EM. This antibody's binding mechanism involves impairing capsid motions and integrity, which inhibits multiple stages of the Norovirus life cycle (Koromyslova & Hansman, 2017; PMID: 29095961).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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