Mfg Part Number
Ab04585-10.3

Vector Labs Ab04585-10.3 Anti-VCAM-1 [HAE-2z], Human IgG1, Fc Silent™, kappa (100 μg)

Quick Overview
The original antibody was raised by immunizing a mouse with domain 1 of human VCAM-1.
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Product Class:Purified
Clone ID:HAE-2z
Heavy Chain Modification:Fc Silent™
Synonyms:VCAM-1; V-CAM 1; INCAM-100; CD106
Amount:100 μg
Application Codes Clone:IF; WB; ELISA
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG1
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:This antibody is specific for human VCAM-1.
Application Notes:The original antibody was used for immunofluorescence on HCAEC. It was able to show the localization of VCAM-1. This antibody was used for a western blot on extracts of HCAEC (Seye et al., 2003; PMID:12714597), and on mesenteric arteries derived from WT and A2BAR-KO mice (Yang et al., 2006; PMID:16823489), and on Mouse microvascular EC (Zerr et al., 2011; PMID:21576651). This antibody was used for an ELISA on HUVECs to show VCAM-1 (Haguet et al., 2020; PMID:32719607). The effect of this antibody on the growth of human blood outgrowth endothelial cells in mice was tested (Milbauer et al., 2009; PMID:19304277).
More Information
Brand Vector Labs
UNSPSC 12161500