Mfg Part Number
Ab04586-1.1

Vector Labs Ab04586-1.1 Anti-CD62L [LAM1-116], Mouse IgG1, kappa (100 μg)

Quick Overview
This original antibody was raised by immunizing a L-selectin deficient mouse with L-selectin.
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Product Class:Purified
Clone ID:LAM1-116
Synonyms:SELL; L-selectin; LAM-1; LECAM1; Leukocyte surface antigen Leu-8; Leukocyte adhesion molecule 1; Lymph node homing receptor; CD62 antigen-like family member L; Leukocyte-endothelial cell adhesion molecule 1
Amount:100 μg
Application Codes Clone:FC; IP; IF; IHC
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG2c
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG2c format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:This antibody is specific for the natural ligand binding domain of L-selectin.
Application Notes:The original antibody was used for flow cytometry on Holstein calve and sheep leukocytes (Leid et al., 2001; PMID:11290767), on EDTA-anticoagulated guinea pig blood (Fryer et al., 1997; PMID:9109449), an d on plasma samples from rats (Yan et al., 2000; PMID:10803628). This antibody was used for immunoprecipitation of L-selectin from bovine lymphocyte lysates (Leid et al., 2001; PMID:11290767), and on RBL cells (Haribabu et al., 1997; PMID:9153259). This antibody was used to test the effect of the antibody on NF-kappa B activation. It was shown that this antibody could indeed activate NF-kappa B in neutrophils (Turutin et al., 2003; PMID:12586371). This antibody was used for immunohistochemistry on a rat endometriosis model and on tissues of human endometriosis. This antibody was able to show the localization of the protein (Odagiri et al., 2007; PMID:17482182). This antibody was used for immunofluorescence on RBL cells where it showed staining like expected (Haribabu et al., 1997; PMID:9153259).
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Brand Vector Labs
UNSPSC 12161500