Mfg Part Number
Ab04600-23.0
Vector Labs Ab04600-23.0 Anti-A2a [Fab2838], Rabbit IgG, kappa (100 μg)
Quick Overview
The original antibody was raised by immunizing a mouse with purified A2AAR-antagonist ZM241385 complex 3.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | Fab2838 |
| Synonyms: | ADORA2; ADORA2A; Adenosine receptor A2a |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; in vitro; crysatallization; blocking |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | Fab |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse Fab format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the intracellular surface of A(2A)AR. |
| Application Notes: | The original antibody was used for an ELISA, where it showed it was able to bind its antigen. The antibody was used for a ligand binding assay. Here it showed that the antibody was able to stop the binding of agonist NECA but not that of antagonist ZM241385. This suggests that this antibody induces a inactive confirmation of A2AAR. The structure of the original antibody was determined using x-ray crystallography (Hino et al., 2012; PMID:22286059). The heavy chain of this antibody was cloned into a plasmid and HEK293T were transfected. In vitro is showed it was able to block the A2AR signalling pathway (Zhang et al., 2019; PMID:32308059). The protein-protein interaction of this antibody with its antigen was assessed using mCBB CN-PAGE (Suzuki et al., 2018; PMID:29432751). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |