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Mfg Part Number

Ab04603-1.1

Vector Labs Ab04603-1.1 Anti-FAS [HFE7A], Mouse IgG1, kappa (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was raised by immunizing a Fas-deficient mice with partially purified human Fas antigen.

$570.70

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Description

Product Class: Purified
Clone ID: HFE7A
Synonyms: CD95; APT1; FAS1; TNFRSF6; Apo-1 antigen; FASLG receptor; Apoptosis-mediating surface antigen FAS; Tumor necrosis factor receptor superfamily member 6
Amount: 100 μg
Application Codes Clone: FC; in vitro; ELISA; crystallization; in vivo
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody is specific for human and mouse FAS.
Application Notes: The original antibody was used for flow cytometry on activated T cells from humans, chimpanzees, Japanese monkeys, crab-eating monkeys and marmosets, and on thymocytes from BALB/c mice. Here it was determined that this antibody was reactive to humans and mice. This antibody was used for a cytotoxic assay on synovial cells (Ichikawa et al., 2000; PMID:10744657). The structure of the original antibody was determined using x-ray crystallography (Ito et al., 2002; PMID:11754745, Haruyama et al., 2002; PMID:12499636). This antibody was used for a competitive ELISA on Fas (Haruyama et al., 2002; PMID:12499636, Yoshida et al., 2010; PMID:20024619). This antibody was used for surface plasmon resonance on mouse Fas, to determine binding affinity. The protective effects of this antibody against acute and lethal hepatic injury induced by Jo2 was assessed. It showed it was able to protect against hepatic injury. To assess the effect of this antibody on caspases in vivo, mice were injected with the antibody after Jo2-induced apoptosis of hepatocytes. It showed that it strongly inhibited activation of caspases (Yoshida et al., 2010; PMID:20024619).

Product Class:	Purified
Clone ID:	HFE7A
Synonyms:	CD95; APT1; FAS1; TNFRSF6; Apo-1 antigen; FASLG receptor; Apoptosis-mediating surface antigen FAS; Tumor necrosis factor receptor superfamily member 6
Amount:	100 μg
Application Codes Clone:	FC; in vitro; ELISA; crystallization; in vivo
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody is specific for human and mouse FAS.
Application Notes:	The original antibody was used for flow cytometry on activated T cells from humans, chimpanzees, Japanese monkeys, crab-eating monkeys and marmosets, and on thymocytes from BALB/c mice. Here it was determined that this antibody was reactive to humans and mice. This antibody was used for a cytotoxic assay on synovial cells (Ichikawa et al., 2000; PMID:10744657). The structure of the original antibody was determined using x-ray crystallography (Ito et al., 2002; PMID:11754745, Haruyama et al., 2002; PMID:12499636). This antibody was used for a competitive ELISA on Fas (Haruyama et al., 2002; PMID:12499636, Yoshida et al., 2010; PMID:20024619). This antibody was used for surface plasmon resonance on mouse Fas, to determine binding affinity. The protective effects of this antibody against acute and lethal hepatic injury induced by Jo2 was assessed. It showed it was able to protect against hepatic injury. To assess the effect of this antibody on caspases in vivo, mice were injected with the antibody after Jo2-induced apoptosis of hepatocytes. It showed that it strongly inhibited activation of caspases (Yoshida et al., 2010; PMID:20024619).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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