Mfg Part Number
Ab04604-23.0

Vector Labs Ab04604-23.0 Anti-ABCB1 [C219], Rabbit IgG, kappa (100 μg)

Quick Overview
The original antibody was raised against plasma membranes of multidrug-resistant Chinese hamster ovary cells.
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Product Class:Purified
Clone ID:C219
Synonyms:CD243; P-glycoprotein 1; PGP1; PGY1; Multidrug resistance protein 1; Phospholipid transporter ABCB1; ATP-dependent translocase ABCB1; ATP-binding cassette sub-family B member 1
Amount:100 μg
Application Codes Clone:WB; crystallization; IHC; ELISA
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:scFv
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:This antibody is specific for the epitope VQ(E/A)ALD of Chinese hamster ABCB1.
Application Notes:The original antibody was used for a western blot on whole cell extracts of E. coli cells overexpressing a fusion of the carboxyl-terminal NBD of Pgp to maltose-binding protein. The structure of the original antibody was determined using x-ray crystallography (Hoedemaeker et al., 1997; PMID:9368049, van Den Elsen et al., 1999; PMID:10570132). This antibody was used for immunohistochemistry on breast cancer cell lines, to determine the cross reactivity of this antibody with p185. This antibody was used for an ELISA on different peptides from p170 and p185 (Liu et al., 1997; PMID:9337349). This antibody was used for immnohistochemistry on high-grade lymphoma from dogs (Bergman et al., 1996; PMID:8947866), and on Ovarian Epithelium, Fallopian Tube, and Ovarian Carcinoma Specimens (Finstad et al., 1991; PMID:1682363).
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Brand Vector Labs
UNSPSC 12161500