Mfg Part Number
Ab04626-10.3
Vector Labs Ab04626-10.3 Anti-Sulphonamide drug [4D11], Human IgG1, Fc Silent™, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing mice with a hapten made by covalently conjugating 4-(4-(4-aminophenylsulfonamido)phenyl)butanoic acid (SA10) with BSA.
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| Product Class: | Purified |
|---|---|
| Clone ID: | 4D11 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | SDM; SMM; SMX; SMZ; SQX; sulfadoxine; sulfamonomethoxine ; sulfamethoxazole; sulfamethazine; sulfaquinoxaline |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody shows high sensitivity for detection of sulfadoxine (SDM), sulfamonomethoxine (SMM), sulfamethoxazole (SMX), sulfamethazine (SMZ), and sulfaquinoxaline (SQX). It had high affinity for sulfisomidine (SIM), SMZ (including acetyl-SMZ), and sulfanitran (SNT). This antibody has higher affinity for the sulfonamides containing a six-atom-ring substitution (except sulfabenzamide -SBA and SDM) rather than a five atom-ring substitution at the N1 position for e.g. sulfamethizole (SMT) and sulfathiazole (STZ). Sulfonamides are synthetic bacteriostatic antibiotics that competitively inhibit conversion of p-aminobenzoic acid to dihydropteroate, which bacteria need for folate synthesis and ultimately purine and DNA synthesis. |
| Application Notes: | The binding reactivity of this antibody for various sulphonamide drugs was determined using ELISA (PMID: 23417550). The binding affinity of this antibody towards various sulphonamide drugs is listed in table 1 of PMID: 30580515. This antibody was used in the development of broad-specific and sensitive competitive indirect ELISA for screening for sulfonamides. It used MAb 4D11 and the BS-BSA as heterologous-coating antigen and demonstrated a 50 % specific binding (IC50) for 22 sulfonamides at concentrations below 100 ng mL−1. The optimized ciELISA was used to quantify the five sulfonamides, SMZ, SDM, SQX, SMM, and SMX in spiked milk samples (Lian et al., Food Anal. Methods (2014)). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |