Vector Labs Ab04626-23.0 Anti-Sulphonamide drug [4D11], Rabbit IgG, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 4D11
Synonyms: SDM; SMM; SMX; SMZ; SQX; sulfadoxine; sulfamonomethoxine ; sulfamethoxazole; sulfamethazine; sulfaquinoxaline
Amount: 100 μg
Application Codes Clone: ELISA
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody shows high sensitivity for detection of sulfadoxine (SDM), sulfamonomethoxine (SMM), sulfamethoxazole (SMX), sulfamethazine (SMZ), and sulfaquinoxaline (SQX). It had high affinity for sulfisomidine (SIM), SMZ (including acetyl-SMZ), and sulfanitran (SNT). This antibody has higher affinity for the sulfonamides containing a six-atom-ring substitution (except sulfabenzamide -SBA and SDM) rather than a five atom-ring substitution at the N1 position for e.g. sulfamethizole (SMT) and sulfathiazole (STZ). Sulfonamides are synthetic bacteriostatic antibiotics that competitively inhibit conversion of p-aminobenzoic acid to dihydropteroate, which bacteria need for folate synthesis and ultimately purine and DNA synthesis.
Application Notes: The binding reactivity of this antibody for various sulphonamide drugs was determined using ELISA (PMID: 23417550). The binding affinity of this antibody towards various sulphonamide drugs is listed in table 1 of PMID: 30580515. This antibody was used in the development of broad-specific and sensitive competitive indirect ELISA for screening for sulfonamides. It used MAb 4D11 and the BS-BSA as heterologous-coating antigen and demonstrated a 50 % specific binding (IC50) for 22 sulfonamides at concentrations below 100 ng mL−1. The optimized ciELISA was used to quantify the five sulfonamides, SMZ, SDM, SQX, SMM, and SMX in spiked milk samples (Lian et al., Food Anal. Methods (2014)).

Product Class:	Purified
Clone ID:	4D11
Synonyms:	SDM; SMM; SMX; SMZ; SQX; sulfadoxine; sulfamonomethoxine ; sulfamethoxazole; sulfamethazine; sulfaquinoxaline
Amount:	100 μg
Application Codes Clone:	ELISA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody shows high sensitivity for detection of sulfadoxine (SDM), sulfamonomethoxine (SMM), sulfamethoxazole (SMX), sulfamethazine (SMZ), and sulfaquinoxaline (SQX). It had high affinity for sulfisomidine (SIM), SMZ (including acetyl-SMZ), and sulfanitran (SNT). This antibody has higher affinity for the sulfonamides containing a six-atom-ring substitution (except sulfabenzamide -SBA and SDM) rather than a five atom-ring substitution at the N1 position for e.g. sulfamethizole (SMT) and sulfathiazole (STZ). Sulfonamides are synthetic bacteriostatic antibiotics that competitively inhibit conversion of p-aminobenzoic acid to dihydropteroate, which bacteria need for folate synthesis and ultimately purine and DNA synthesis.
Application Notes:	The binding reactivity of this antibody for various sulphonamide drugs was determined using ELISA (PMID: 23417550). The binding affinity of this antibody towards various sulphonamide drugs is listed in table 1 of PMID: 30580515. This antibody was used in the development of broad-specific and sensitive competitive indirect ELISA for screening for sulfonamides. It used MAb 4D11 and the BS-BSA as heterologous-coating antigen and demonstrated a 50 % specific binding (IC50) for 22 sulfonamides at concentrations below 100 ng mL−1. The optimized ciELISA was used to quantify the five sulfonamides, SMZ, SDM, SQX, SMM, and SMX in spiked milk samples (Lian et al., Food Anal. Methods (2014)).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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