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Mfg Part Number

Ab04635-1.29

Vector Labs Ab04635-1.29 Anti-THC [T3], Mouse Fab fragment, His-Tagged, kappa (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing mice a THC-BSA conjugate.

$692.90

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Description

Product Class: Purified
Clone ID: T3
Heavy Chain Modification: His-Tagged
Synonyms: Δ9-THC
Amount: 100 μg
Application Codes Clone: ELISA; Functional assays; Crystallization
Shipping Temperature: Wet Ice
Origin Pub PMID: 20630472
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: Fab
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This reformatted mouse antibody was made using the variable domain sequences of the original Mouse Fab format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for Δ9-THC and its metabolites, including 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (11-nor-Δ9-THC—COOH) and 11‑hydroxy-Δ9-tetrahydrocannabinol (11-OH-Δ9-THC).
Application Notes: The binding affinity and specificity of the original version of this antibody (mouse Fab) were determined in a competitive ELISA. Structural analysis of this antibody — free and in complex with THC — was determined through crystallization. This antibody was also used to develop a sensitive, one-step, homogeneous FRET immunoassay for detecting THC and its metabolites in saliva samples (Niemi et al., 2010; PMID: 20630472) (US8518653B2). This antibody was successfully transiently expressed in N. benthamiana by using a geminiviral expression system. The plant-produced version of this antibody retained the binding activity to Δ9-THC and its metabolites and provided high sensitivity in detecting Δ9-THC in marijuana, inflorescence, and leaf extracts but not in stem, root, or non-cannabis controls (Boonyayothin et al., 2022; PMID: 35686006).

Product Class:	Purified
Clone ID:	T3
Heavy Chain Modification:	His-Tagged
Synonyms:	Δ9-THC
Amount:	100 μg
Application Codes Clone:	ELISA; Functional assays; Crystallization
Shipping Temperature:	Wet Ice
Origin Pub PMID:	20630472
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	Fab
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This reformatted mouse antibody was made using the variable domain sequences of the original Mouse Fab format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for Δ9-THC and its metabolites, including 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (11-nor-Δ9-THC—COOH) and 11‑hydroxy-Δ9-tetrahydrocannabinol (11-OH-Δ9-THC).
Application Notes:	The binding affinity and specificity of the original version of this antibody (mouse Fab) were determined in a competitive ELISA. Structural analysis of this antibody — free and in complex with THC — was determined through crystallization. This antibody was also used to develop a sensitive, one-step, homogeneous FRET immunoassay for detecting THC and its metabolites in saliva samples (Niemi et al., 2010; PMID: 20630472) (US8518653B2). This antibody was successfully transiently expressed in N. benthamiana by using a geminiviral expression system. The plant-produced version of this antibody retained the binding activity to Δ9-THC and its metabolites and provided high sensitivity in detecting Δ9-THC in marijuana, inflorescence, and leaf extracts but not in stem, root, or non-cannabis controls (Boonyayothin et al., 2022; PMID: 35686006).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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