Mfg Part Number
Ab04639-30.11
Vector Labs Ab04639-30.11 Anti-Pep27 [F9], scFv fragment (His), ScFv, (100 μg)
Quick Overview
The original antibody was selected from a synthetic human scFv library encoding HA-tagged scFv clones through five rounds of biopanning against synthetic and recombinant Pep27.
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| Product Class: | Purified |
|---|---|
| Clone ID: | F9 |
| Heavy Chain Modification: | ScFv |
| Synonyms: | Pep27 protein |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; IB |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 29753735 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody is specific for the Streptococcus pneumoniae Pep27. The epitope recognized by this antibody is located within the residues 18-27, with key residues crucial for the binding being Y24, R26, and K27, as identified through alanine scanning and further supported by molecular docking analyses. Streptococcus pneumoniae Pep27 is a small, secreted peptide consisting of 27 amino acids initially described as a "death signal peptide". |
| Application Notes: | The dissociation constant (Kd) of the original human scFv version of this antibody was 0.5 µM as measured in an ELISA. This antibody specifically detected Pep27 in an immunoblot and in an environment mimicking in vivo conditions, demonstrated in human serum (Kim et al. 2018; PMID: 29753735). This antibody was successfully converted and expressed as a Fab, scFv-Fc, scFv-CH, and IgG using an antibody reformatting vector system (AbVec) (Kim, Park, & Lee, 2019). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |