Mfg Part Number
Ab04642-23.159
Vector Labs Ab04642-23.159 Anti-IgG-Fc Domain [IgG-Fc-16], Rabbit IgG-Fc Fusion, (100 μg)
Quick Overview
The original antibody was generated by immunizing llamas with mammalian IgG antibodies and Fc fragments, creating the appropriate phage display library, and panning against the original target.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | IgG-Fc-16 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; SPR |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | VHH |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Llama VHH format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the mammalian IgG-Fc domain. It was tested for and found to bind the human IgG-Fc domain (all subclasses). In a cross-reactivity test, it also bound bovine, mouse, rat, rabbit, dog, cat, swine, sheep, Chimpanzee, Rhesus macaque, donkey, horse, goat, Syrian hamster, and guinea pig IgG-Fcs. |
| Application Notes: | The reactivity of the original version of this antibody (camelid (llama) VHH) was tested using ELISA. Its binding affinity (KD) was measured using SPR and was found to be 13.1, 6.81, 16.2, 21.3, 1.70, 2.79, and 8.41 nM for human IgG, human IgG1, human IgG2, human IgG3, human IgG4, bovine IgG, and mouse IgG, respectively. Its binding to human, mouse, and bovine IgG was further verified via chromatography testing; the dynamic binding capacity of the antibody when immobilized onto NHS sepharose was 11.5 mg human IgG/ml matrix (US9040666B2). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |