Mfg Part Number
Ab04642-34.11

Vector Labs Ab04642-34.11 Anti-IgG-Fc Domain [IgG-Fc-16], Camelid VHH, His-tagged, (100 μg)

Quick Overview
The original antibody was generated by immunizing llamas with mammalian IgG antibodies and Fc fragments, creating the appropriate phage display library, and panning against the original target.
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Product Class:Purified
Clone ID:IgG-Fc-16
Heavy Chain Modification:His-tagged
Amount:100 μg
Application Codes Clone:ELISA; SPR
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:VHH
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:This antibody is specific for the mammalian IgG-Fc domain. It was tested for and found to bind the human IgG-Fc domain (all subclasses). In a cross-reactivity test, it also bound bovine, mouse, rat, rabbit, dog, cat, swine, sheep, Chimpanzee, Rhesus macaque, donkey, horse, goat, Syrian hamster, and guinea pig IgG-Fcs.
Application Notes:The reactivity of the original version of this antibody (camelid (llama) VHH) was tested using ELISA. Its binding affinity (KD) was measured using SPR and was found to be 13.1, 6.81, 16.2, 21.3, 1.70, 2.79, and 8.41 nM for human IgG, human IgG1, human IgG2, human IgG3, human IgG4, bovine IgG, and mouse IgG, respectively. Its binding to human, mouse, and bovine IgG was further verified via chromatography testing; the dynamic binding capacity of the antibody when immobilized onto NHS sepharose was 11.5 mg human IgG/ml matrix (US9040666B2).
More Information
Brand Vector Labs
UNSPSC 12161500