Vector Labs Ab04661-3.0 Anti-ST2L [ST2M51], Mouse IgG2b, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: ST2M51
Synonyms: ST2; IL1RL1; IL-1RL1; DER4; T1; IL-33Rα; Interleukin-1 receptor-like 1; Protein ST2; Growth Stimulation Expressed Gene 2; suppression of tumorigenicity 2
Amount: 100 μg
Application Codes Clone: ELISA; FC; functional assay
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG2
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG2 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody specifically binds an epitope in domain III of the human ST2L and cross reacts with cynomolgus monkey ST2L. ST2L a transmembrane receptor that belongs to the IL-1 receptor family which is expressed on the cell surface of a wide variety of immune cells including T cell, NK/NKT cells, basophils, eosinophils, mast cells and the newly-described non-B/non-T innate lymphoid type 2 cells, nuocytes, and natural helper cells. ST2L expression is also inducible on dendritic cells (DCs), macrophages, and neutrophils. ST2L is able to downregulate the responsiveness of Toll-like Receptors TLR2, TLR4, and TLR9, but also induce type 2 cytokine release via activation by its ligand IL-33 and association with accessory protein IL-1RAcP.
Application Notes: This antibody is not capable of blocking the interaction between IL-33 and ST2L. This antibody was capable of recognizing recombinant human ST2L extracellular domain (ST2L-ECD) using an ELISA assay (PMID: 27294560). The original human IgG2 chimeric version of the antibody binds human and cynomolgus monkey ST2L with a binding affinity of 38 pM and 76 pM respectively (US9090694). This antibody was capable of binding ST2L expressed by LAD2 mast cells in a flow cytometric assay. This antibody was reported to block the IL-33 induced activation of NF-kB in a dose dependent manner. This antibody also blocked IL-1RAcP from binding to the ST2L/IL-33 complex. It can also inhibit cytokine secretion by human basophilic cell line KU812 (PMID: 27294560).

Product Class:	Purified
Clone ID:	ST2M51
Synonyms:	ST2; IL1RL1; IL-1RL1; DER4; T1; IL-33Rα; Interleukin-1 receptor-like 1; Protein ST2; Growth Stimulation Expressed Gene 2; suppression of tumorigenicity 2
Amount:	100 μg
Application Codes Clone:	ELISA; FC; functional assay
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG2
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG2 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody specifically binds an epitope in domain III of the human ST2L and cross reacts with cynomolgus monkey ST2L. ST2L a transmembrane receptor that belongs to the IL-1 receptor family which is expressed on the cell surface of a wide variety of immune cells including T cell, NK/NKT cells, basophils, eosinophils, mast cells and the newly-described non-B/non-T innate lymphoid type 2 cells, nuocytes, and natural helper cells. ST2L expression is also inducible on dendritic cells (DCs), macrophages, and neutrophils. ST2L is able to downregulate the responsiveness of Toll-like Receptors TLR2, TLR4, and TLR9, but also induce type 2 cytokine release via activation by its ligand IL-33 and association with accessory protein IL-1RAcP.
Application Notes:	This antibody is not capable of blocking the interaction between IL-33 and ST2L. This antibody was capable of recognizing recombinant human ST2L extracellular domain (ST2L-ECD) using an ELISA assay (PMID: 27294560). The original human IgG2 chimeric version of the antibody binds human and cynomolgus monkey ST2L with a binding affinity of 38 pM and 76 pM respectively (US9090694). This antibody was capable of binding ST2L expressed by LAD2 mast cells in a flow cytometric assay. This antibody was reported to block the IL-33 induced activation of NF-kB in a dose dependent manner. This antibody also blocked IL-1RAcP from binding to the ST2L/IL-33 complex. It can also inhibit cytokine secretion by human basophilic cell line KU812 (PMID: 27294560).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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