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Mfg Part Number

Ab04693-23.0

Vector Labs Ab04693-23.0 Anti-HTT-N17 [scFv-C4], Rabbit IgG, lambda (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was isolated screening a naïve human single-chain Fv antibody phage-display library against HTT(1-17).

$570.70

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Description

Product Class: Purified
Clone ID: scFv-C4
Synonyms: C4; HTT; Huntingtin; HD protein; Huntington disease protein
Amount: 100 μg
Application Codes Clone: ELISA; IF; SPR; in vitro; crystallization
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: scFv
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: The antibody is specific for huntingtin. It binds to the first 17 residues of huntingtin [HTT(1-17)].
Application Notes: The original format of the antibody could inhibit the aggregation of mHTT-exon1 protein fragments in vitro. The crystal structure of the antibody in complex with HTT(1-17) was determined (De Genst et al., 2015; PMID: 25861763). The specificity of the antibody was confirmed by ELISA analysis. The original format of the antibody-targeted the HD-Q103-myc antigen to cell nuclei in COS-7 cells by immunofluorescence microscopy. The binding affinity of the antibody to HTT(1-17) was measured by surface plasmon resonance (Kd= 7.9 nM) (Lecerf et al., 2001; PMID: 11296304). The antibody selectively bound diffuse htt, but not aggregated htt, as shown by immunofluorescence analysis. Immunoprecipitation experiments in stably transfected, intrabody-expressing STHdhQ111 cells demonstrated that the antibody bound a small fraction of wild-type and mutant endogenous full-length htt (Miller et al., 2005; PMID: 15837560).

Product Class:	Purified
Clone ID:	scFv-C4
Synonyms:	C4; HTT; Huntingtin; HD protein; Huntington disease protein
Amount:	100 μg
Application Codes Clone:	ELISA; IF; SPR; in vitro; crystallization
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	scFv
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	The antibody is specific for huntingtin. It binds to the first 17 residues of huntingtin [HTT(1-17)].
Application Notes:	The original format of the antibody could inhibit the aggregation of mHTT-exon1 protein fragments in vitro. The crystal structure of the antibody in complex with HTT(1-17) was determined (De Genst et al., 2015; PMID: 25861763). The specificity of the antibody was confirmed by ELISA analysis. The original format of the antibody-targeted the HD-Q103-myc antigen to cell nuclei in COS-7 cells by immunofluorescence microscopy. The binding affinity of the antibody to HTT(1-17) was measured by surface plasmon resonance (Kd= 7.9 nM) (Lecerf et al., 2001; PMID: 11296304). The antibody selectively bound diffuse htt, but not aggregated htt, as shown by immunofluorescence analysis. Immunoprecipitation experiments in stably transfected, intrabody-expressing STHdhQ111 cells demonstrated that the antibody bound a small fraction of wild-type and mutant endogenous full-length htt (Miller et al., 2005; PMID: 15837560).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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