Mfg Part Number
Ab04694-23.0
Vector Labs Ab04694-23.0 Anti-SpuE [ScFv5], Rabbit IgG, lambda (100 μg)
Quick Overview
The original antibody was isolated from a human scFv phage-displayed library by panning against SpuE.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | ScFv5 |
| Synonyms: | Spermidine-binding periplasmic protein SpuE |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; Crystallization |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | The antibody binds specifically to SpuE. In particular, it interacts with the hinge region that connects the two domains of SpuE. |
| Application Notes: | The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody conjugated to a membrane penetrating peptide (MPP) could inhibit the secretion of ExoS from P. aeruginosa. The antibody conjugated to MPP was able to inhibit the cytotoxic effects of SpuE in the human epithelial cell line A549. The antibody conjugated to MPP showed a protective effect on C. elegans from P. aeruginosa infection. The crystal structure of the antibody in complex with SpuE-spermidine was determined. The dissociation constant (KD) of SpuE to spermidine and of SpuE to spermidine in the presence of the antibody was measured by isothermal titration calorimetry (Kd = 2.7 nM and 29.8 nM, respectively) (Zhang et al., 2019; PMID: 31682834). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |