Mfg Part Number
Ab04720-10.3
Vector Labs Ab04720-10.3 Anti-GM1-bound Aβ [4396], Human IgG1, Fc Silent™, lambda (100 μg)
Quick Overview
The original antibody was generated by immunizing mice with purified human brain-derived GM1-Aβ.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | 4396 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | Gaβ; GM1 ganglioside-bound amyloid β-protein; 4396C |
| Amount: | 100 μg |
| Application Codes Clone: | EM; WB; ThT fluorescence assay; IM |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgM |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for human GM1-Aβ. |
| Application Notes: | The original mouse IgM antibody was developed and tested to specifically recognize Aβ bound to GM1 (GM1-Aβ). A subsequent version, 4396C, an IgG2a subtype chimeric antibody, was generated and evaluated. The chimeric antibody retained its specificity for GM1-Aβ, as demonstrated by electron microscopy (EM), where it specifically labeled GM1-containing liposomes incubated with Aβ; Western blotting (WB), which confirmed the antibody’s specificity for GM1-Aβ; a Thioflavin T (ThT) assay, which showed that the antibody inhibited amyloid fibril formation in a dose-dependent manner when incubated with GM1-containing liposomes and Aβ; and immunoelectron microscopy (IM), where it effectively bound to newly formed amyloid fibrils in the presence of synthetic Aβ and GM1-containing liposomes (US7339035B2). The chimeric antibody was assessed in three polymerization systems: (1) polymerization initiated by adding Aβ fibril fragment, (2) Aβ40 polymerization initiated from GAβ40, and (3) Aβ42 polymerization initiated from GAβ42. It exhibited suppression of the Aβ extension (US7741448B2). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |