Vector Labs Ab04722-10.3 Anti-NeuGcGM3 [P3], Human IgG1, Fc Silent™, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: P3
Heavy Chain Modification: Fc Silent™
Synonyms: NeuGcGM2; N-glycolyl neuraminic acid-containing gangliosides; NeuGc-containing gangliosides; GM3(NeuGc) ganglioside
Amount: 100 μg
Application Codes Clone: ELISA; HPTLC; IHC; in vivo
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgM
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for the N-glycolyl neuraminic acid (NeuGc) linked to the internal galactose of gangliosides, showing no cross-reactivity to the highly similar N-acetylated (NeuAc) gangliosides that are common cellular antigens in humans. This antibody was shown to react strongly with NeuGcGM3 and NeuGcGM2, but not with GM3, GM2, GM1, GD1a, GD1b, GD3, GD2, GT1b, Gg4Cer, or LacCer. This antibody also binds sulfated glycolipids.
Application Notes: The original antibody (mouse IgM) was generated and screened via ELISA against for gangliosides of interest. This antibody was used for tumor recognition in IHC, showing a strong positive reaction in ductal infiltrating breast carcinoma, breast cancer metastasis in lymph nodes, and breast adenosis tissue, with no cross-reactivity to other malignant or benign tumors. Additionally, it was used in in vivo experiments with Balb/c mice to stimulate immune responses, generating a significant IgG-type anti-idiotypic (Ab2) response, which increased when conjugated to KLH and administered with adjuvants. This antibody also produced an anti-anti-idiotypic (Ab1') response, demonstrating its ability to connect to both T and B cell networks and generate a specific antigen-independent immune response (EP0657471B1). This antibody was used as a positive control in an in vitro and in vivo study (Carr et al., 2002; PMID: 12573110). A chimeric(humanized; human IgG1) variant of this antibody was created (chP3), and the crystal structure of its Fab was solved (Talavera et al., 2009; PMID: 19748674).

Product Class:	Purified
Clone ID:	P3
Heavy Chain Modification:	Fc Silent™
Synonyms:	NeuGcGM2; N-glycolyl neuraminic acid-containing gangliosides; NeuGc-containing gangliosides; GM3(NeuGc) ganglioside
Amount:	100 μg
Application Codes Clone:	ELISA; HPTLC; IHC; in vivo
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgM
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for the N-glycolyl neuraminic acid (NeuGc) linked to the internal galactose of gangliosides, showing no cross-reactivity to the highly similar N-acetylated (NeuAc) gangliosides that are common cellular antigens in humans. This antibody was shown to react strongly with NeuGcGM3 and NeuGcGM2, but not with GM3, GM2, GM1, GD1a, GD1b, GD3, GD2, GT1b, Gg4Cer, or LacCer. This antibody also binds sulfated glycolipids.
Application Notes:	The original antibody (mouse IgM) was generated and screened via ELISA against for gangliosides of interest. This antibody was used for tumor recognition in IHC, showing a strong positive reaction in ductal infiltrating breast carcinoma, breast cancer metastasis in lymph nodes, and breast adenosis tissue, with no cross-reactivity to other malignant or benign tumors. Additionally, it was used in in vivo experiments with Balb/c mice to stimulate immune responses, generating a significant IgG-type anti-idiotypic (Ab2) response, which increased when conjugated to KLH and administered with adjuvants. This antibody also produced an anti-anti-idiotypic (Ab1') response, demonstrating its ability to connect to both T and B cell networks and generate a specific antigen-independent immune response (EP0657471B1). This antibody was used as a positive control in an in vitro and in vivo study (Carr et al., 2002; PMID: 12573110). A chimeric(humanized; human IgG1) variant of this antibody was created (chP3), and the crystal structure of its Fab was solved (Talavera et al., 2009; PMID: 19748674).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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