JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser.

Skip to the beginning of the images gallery

Mfg Part Number

Ab04726-10.3

Vector Labs Ab04726-10.3 Anti-tumor-associated gangliosides [PolrecCHOIgM/GD3], Human IgG1, Fc Silent™, kappa (100 μg)

VECTOR LABS

Be the first to review this product

Quick Overview

The original antibody was developed by isolating heavy and light chain cDNAs from a hybridoma producing an anti-GD3 IgM antibody. These sequences were then codon-optimized and transfected into CHO cells to produce the recombinant PolrecCHOIgM/GD3 antibody.

$570.70

Order will ship within 1-3 business days

Description

Product Class: Purified
Clone ID: PolrecCHOIgM/GD3
Heavy Chain Modification: Fc Silent™
Synonyms: GD3; GM3; GD2; GM1
Amount: 100 μg
Application Codes Clone: ELISA; IF; in vitro; Functional assays
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgM
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This reformatted human antibody was made using the variable domain sequences of the original Human IgM format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for human GD3, GM1, GD2, and GM3 gangliosides.
Application Notes: The original antibody (recombinant human IgM) was designed to bind the ganglioside GD3, which is overexpressed in malignant cells like melanomas. ELISA testing revealed strong binding specificity to GD3, GM1, GD2, and GM3 gangliosides, even at low concentrations. In cell-line assays, it selectively bound cancer cell lines (melanoma, lung, breast, and glioblastoma), without binding to normal cells such as huVEC and lung fibroblasts. Immunoimaging confirmed this selective binding, with fluorescence indicating strong interaction with malignant cells. In vitro complement activation tests showed that the antibody triggered cancer cell lysis when exposed to normal human serum, demonstrating its ability to activate the complement cascade. The antibody thus effectively discriminates between malignant and normal cells, confirming its diagnostic and therapeutic utility in cancer (US10590204B2).

Product Class:	Purified
Clone ID:	PolrecCHOIgM/GD3
Heavy Chain Modification:	Fc Silent™
Synonyms:	GD3; GM3; GD2; GM1
Amount:	100 μg
Application Codes Clone:	ELISA; IF; in vitro; Functional assays
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgM
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This reformatted human antibody was made using the variable domain sequences of the original Human IgM format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for human GD3, GM1, GD2, and GM3 gangliosides.
Application Notes:	The original antibody (recombinant human IgM) was designed to bind the ganglioside GD3, which is overexpressed in malignant cells like melanomas. ELISA testing revealed strong binding specificity to GD3, GM1, GD2, and GM3 gangliosides, even at low concentrations. In cell-line assays, it selectively bound cancer cell lines (melanoma, lung, breast, and glioblastoma), without binding to normal cells such as huVEC and lung fibroblasts. Immunoimaging confirmed this selective binding, with fluorescence indicating strong interaction with malignant cells. In vitro complement activation tests showed that the antibody triggered cancer cell lysis when exposed to normal human serum, demonstrating its ability to activate the complement cascade. The antibody thus effectively discriminates between malignant and normal cells, confirming its diagnostic and therapeutic utility in cancer (US10590204B2).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

Reviews