Mfg Part Number
Ab04753-23.0
Vector Labs Ab04753-23.0 Anti-EGFR [EGFR-7], Rabbit IgG, kappa (100 μg)
Quick Overview
The original antibody was raised by immunizing BALB/c mice with head and neck squamous carcinoma cell lines CA922 and HSC4.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | EGFR-7 |
| Synonyms: | ERBB; ERBB1; HER1; Proto-oncogene c-ErbB-1; Epidermal growth factor receptor; Receptor tyrosine-protein kinase erbB-1 |
| Amount: | 100 μg |
| Application Codes Clone: | FC; WB; in vivo; in vitro |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for human EGFR. |
| Application Notes: | The original antibody was used for flow cytometry on cell either untreated or treated with EGF. This antibody was used for a western blot on MDA-MB468 tumor cell line and human primary adult keratinocytes. This antibody was used for a proliferation assay on H292 tumor cells, it showed a EC50 of 0.21 nM. A humanized version of this antibody was used in vivo for H292 and FaDu tumor xenografts, here it showed it was very active and delayed the tumor growth (US8790649B2). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |