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Mfg Part Number

Ab04760-23.0

Vector Labs Ab04760-23.0 Anti-CNN1 [Mab420], Rabbit IgG, lambda (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was raised by immunizing a BALB/c or NMRI mouse with recombinant human CCN1 containing the single amino acid exchange E173D or F185L

$570.70

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Description

Product Class: Purified
Clone ID: Mab420
Synonyms: IBP-10; Protein GIG1; Protein CYR61; IGF-binding protein 10; IGFBP-10; CCN family member 1; Cysteine-rich angiogenic inducer 61; Insulin-like growth factor-binding protein 10
Amount: 100 μg
Application Codes Clone: FC; ELISA; SPR; IF; in vitro; in vivo
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: Fab
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for human and cynomolgus monkey CNN1.
Application Notes: The original antibody was used for flow cytometry on suspension adapted HEK293 cells. In a further flow cytometry experiment it was shown that the antibody also bound to cynomolgus monkey CNN1 This antibody was used for an ELISA on both human and mouse CNN1. The binding affinity of this antibody to human CNN1 was determined using surface plasmon resonance. It showed a Kd of 3.02 * 10^-8. This antibody was used for immunofluorescence on NIH 3T3 cells or suspension adapted HEK293 cells transfected with the expression plasmids driving the expression of the CCN1. It showed it could correctly label the CNN1 protein in vitro. This antibody was used in vivo on different tumor cell lines xenografted into a SCID-beige mice. It was effective for SKOV3 cells and Panc-1 cells but not MDA-MB-435 (US20120258112A1).

Product Class:	Purified
Clone ID:	Mab420
Synonyms:	IBP-10; Protein GIG1; Protein CYR61; IGF-binding protein 10; IGFBP-10; CCN family member 1; Cysteine-rich angiogenic inducer 61; Insulin-like growth factor-binding protein 10
Amount:	100 μg
Application Codes Clone:	FC; ELISA; SPR; IF; in vitro; in vivo
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	Fab
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for human and cynomolgus monkey CNN1.
Application Notes:	The original antibody was used for flow cytometry on suspension adapted HEK293 cells. In a further flow cytometry experiment it was shown that the antibody also bound to cynomolgus monkey CNN1 This antibody was used for an ELISA on both human and mouse CNN1. The binding affinity of this antibody to human CNN1 was determined using surface plasmon resonance. It showed a Kd of 3.02 * 10^-8. This antibody was used for immunofluorescence on NIH 3T3 cells or suspension adapted HEK293 cells transfected with the expression plasmids driving the expression of the CCN1. It showed it could correctly label the CNN1 protein in vitro. This antibody was used in vivo on different tumor cell lines xenografted into a SCID-beige mice. It was effective for SKOV3 cells and Panc-1 cells but not MDA-MB-435 (US20120258112A1).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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