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Mfg Part Number

Ab04761-10.3

Vector Labs Ab04761-10.3 Anti-CD266 [P4A8], Human IgG1, Fc Silent™, kappa (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was raised by immunizing Fn14-deficient mice with CHO cells expressing human surface Fn14 and boosted with Fn14-myc-His

$570.70

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Description

Product Class: Purified
Clone ID: P4A8
Heavy Chain Modification: Fc Silent™
Synonyms: FN14; TweakR; TNFRSF12A; FGF-inducible 14; Tweak-receptor; Tumor necrosis factor receptor superfamily member 12A; Fibroblast growth factor-inducible immediate-early response protein 14
Amount: 100 μg
Application Codes Clone: inhibition; FC; WB; IHC
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for human Fn14.
Application Notes: The original antibody was tested to inhibit distinct TWEAK-induced cellular effects known to be mediated by Fn14. HT29 and WiDr cells were treated with this antibody, this resulted in a strong inhibition of TWEAK-induced IL8 production. Furthermore, it was shown that oligomerization can greatly increase the agonistic activity of this antibody (Salzmann et al., 2013; PMID:23532848). This antibody was used for flow cytometry on HEK293 cells transfected with plasmids expressing Fn14 protein. This antibody was used for a western blot on lysates from HEK293 cells expressing Fn14 (Trebing et al., 2014; PMID:24135629). This antibody was used for immunohistochemistry on different human brain tumors (Tran et al., 2006; PMID:17018610), and on samples from xenografted tumors made from H460 cells (Jandova et al., 2015; PMID:25563366).

Product Class:	Purified
Clone ID:	P4A8
Heavy Chain Modification:	Fc Silent™
Synonyms:	FN14; TweakR; TNFRSF12A; FGF-inducible 14; Tweak-receptor; Tumor necrosis factor receptor superfamily member 12A; Fibroblast growth factor-inducible immediate-early response protein 14
Amount:	100 μg
Application Codes Clone:	inhibition; FC; WB; IHC
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for human Fn14.
Application Notes:	The original antibody was tested to inhibit distinct TWEAK-induced cellular effects known to be mediated by Fn14. HT29 and WiDr cells were treated with this antibody, this resulted in a strong inhibition of TWEAK-induced IL8 production. Furthermore, it was shown that oligomerization can greatly increase the agonistic activity of this antibody (Salzmann et al., 2013; PMID:23532848). This antibody was used for flow cytometry on HEK293 cells transfected with plasmids expressing Fn14 protein. This antibody was used for a western blot on lysates from HEK293 cells expressing Fn14 (Trebing et al., 2014; PMID:24135629). This antibody was used for immunohistochemistry on different human brain tumors (Tran et al., 2006; PMID:17018610), and on samples from xenografted tumors made from H460 cells (Jandova et al., 2015; PMID:25563366).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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