Vector Labs Ab04773-10.0 Anti-Aβ1–42 amyloid beta [1E8], Human IgG1, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 1E8
Synonyms: Aβ; CVAP; PN-II; Abeta; Amyloid-beta precursor protein; APP; ABPP; APPI; Alzheimer disease amyloid A4 protein homolog; Alzheimer disease amyloid protein; Amyloid precursor protein; Amyloid-beta precursor protein; Amyloid-beta A4 protein; Cerebral vascular amyloid peptide; PreA4; Protease nexin-II
Amount: 100 μg
Application Codes Clone: ELISA; SPR; IHC; WB; in vitro
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: The antibody targets the central region of amyloid beta (residues 17–22).
Application Notes: The specificity of the original, Fab and scFv formats of the antibody was confirmed by ELISA analysis (Tammer et al., 2002; PMID: 12197886, Nisbet et al., 2013; PMID: 23766374). The original format of the antibody is extensively used for immunohistochemistry for detection of Aβ plaques. The original format and the Fab fragment bound to Ab[1–40/42/43] peptides by western blot analysis. The Fab format of the antibody shown to bind plaques within sections of human AD brain tissue by immunohistochemistry (Tammer et al., 2002; PMID: 12197886). The scFv format of the antibody bound to the central region of Amyloid beta with an affinity of ∼55 nM and significantly reduced fibril formation of Amyloid beta 1–42. Furthermore, the scFv format of the antibody ameliorates Ab1–42-mediated toxicity in the PC12 cell line and murine primary neuronal cultures (Nisbet et al., 2013; PMID: 23766374). The antibody was able to immunoprecipitate aliquots of individual EDTA-blood plasma samples from five different donors by magnetic bead immunoprecipitation (Klafki et al., 2020; PMID: 32911706).

Product Class:	Purified
Clone ID:	1E8
Synonyms:	Aβ; CVAP; PN-II; Abeta; Amyloid-beta precursor protein; APP; ABPP; APPI; Alzheimer disease amyloid A4 protein homolog; Alzheimer disease amyloid protein; Amyloid precursor protein; Amyloid-beta precursor protein; Amyloid-beta A4 protein; Cerebral vascular amyloid peptide; PreA4; Protease nexin-II
Amount:	100 μg
Application Codes Clone:	ELISA; SPR; IHC; WB; in vitro
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	The antibody targets the central region of amyloid beta (residues 17–22).
Application Notes:	The specificity of the original, Fab and scFv formats of the antibody was confirmed by ELISA analysis (Tammer et al., 2002; PMID: 12197886, Nisbet et al., 2013; PMID: 23766374). The original format of the antibody is extensively used for immunohistochemistry for detection of Aβ plaques. The original format and the Fab fragment bound to Ab[1–40/42/43] peptides by western blot analysis. The Fab format of the antibody shown to bind plaques within sections of human AD brain tissue by immunohistochemistry (Tammer et al., 2002; PMID: 12197886). The scFv format of the antibody bound to the central region of Amyloid beta with an affinity of ∼55 nM and significantly reduced fibril formation of Amyloid beta 1–42. Furthermore, the scFv format of the antibody ameliorates Ab1–42-mediated toxicity in the PC12 cell line and murine primary neuronal cultures (Nisbet et al., 2013; PMID: 23766374). The antibody was able to immunoprecipitate aliquots of individual EDTA-blood plasma samples from five different donors by magnetic bead immunoprecipitation (Klafki et al., 2020; PMID: 32911706).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

Reviews