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Mfg Part Number

Ab04787-23.0

Vector Labs Ab04787-23.0 Anti-Carbonic anhydrase IX [G10], Rabbit IgG, lambda (100 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by panning a non-immune human scFv-phage display library against CA IX-expressing cells (293T-G250 cells).

$570.70

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Description

Product Class: Purified
Clone ID: G10
Synonyms: CA9; Carbonic anhydrase 9; Carbonate dehydratase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250; pMW1 MN/CA IX; endogenous protein p54/58N; EC:4.2.1.1
Amount: 100 μg
Application Codes Clone: ELISA; Inhibition; FC; in vivo
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: scFv
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for human CA IX.
Application Notes: The specificity of this antibody's original format (human scFv) was validated via ELISA, demonstrating its selective binding to CA IX-expressing cells. It was further confirmed through flow cytometry and cross-competition studies. This antibody exhibited an IC₅₀ of 2.086 µg/ml / 2.9 nM to stable G250-expressing 293T cells. This antibody was tested in vivo in Ncr Nude mice (athymic mice); it was incorporated into lentiviral vectors to create anti-CA IX chimeric immune receptors for T-lymphocyte transduction, resulting in enhanced T-lymphocyte cytotoxicity against renal cell carcinoma (RCC) cells. An scFv-Fc fusion version of this antibody was successfully used in an internalization study of anti-CA IX scFv-Fc antibody fusion proteins into stable G250-293T cells, exhibiting mild (~20%) inhibition (US8466263B2).

Product Class:	Purified
Clone ID:	G10
Synonyms:	CA9; Carbonic anhydrase 9; Carbonate dehydratase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250; pMW1 MN/CA IX; endogenous protein p54/58N; EC:4.2.1.1
Amount:	100 μg
Application Codes Clone:	ELISA; Inhibition; FC; in vivo
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	scFv
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for human CA IX.
Application Notes:	The specificity of this antibody's original format (human scFv) was validated via ELISA, demonstrating its selective binding to CA IX-expressing cells. It was further confirmed through flow cytometry and cross-competition studies. This antibody exhibited an IC₅₀ of 2.086 µg/ml / 2.9 nM to stable G250-expressing 293T cells. This antibody was tested in vivo in Ncr Nude mice (athymic mice); it was incorporated into lentiviral vectors to create anti-CA IX chimeric immune receptors for T-lymphocyte transduction, resulting in enhanced T-lymphocyte cytotoxicity against renal cell carcinoma (RCC) cells. An scFv-Fc fusion version of this antibody was successfully used in an internalization study of anti-CA IX scFv-Fc antibody fusion proteins into stable G250-293T cells, exhibiting mild (~20%) inhibition (US8466263B2).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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