Mfg Part Number
Ab04787-30.11
Vector Labs Ab04787-30.11 Anti-Carbonic anhydrase IX [G10], scFv fragment (His), ScFv, (100 μg)
Quick Overview
The original antibody was generated by panning a non-immune human scFv-phage display library against CA IX-expressing cells (293T-G250 cells).
$692.90
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | G10 |
| Heavy Chain Modification: | ScFv |
| Synonyms: | CA9; Carbonic anhydrase 9; Carbonate dehydratase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250; pMW1 MN/CA IX; endogenous protein p54/58N; EC:4.2.1.1 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; Inhibition; FC; in vivo |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | This antibody is specific for human CA IX. |
| Application Notes: | The specificity of this antibody's original format (human scFv) was validated via ELISA, demonstrating its selective binding to CA IX-expressing cells. It was further confirmed through flow cytometry and cross-competition studies. This antibody exhibited an IC50 of 2.086 µg/ml / 2.9 nM to stable G250-expressing 293T cells. This antibody was tested in vivo in Ncr Nude mice (athymic mice); it was incorporated into lentiviral vectors to create anti-CA IX chimeric immune receptors for T-lymphocyte transduction, resulting in enhanced T-lymphocyte cytotoxicity against renal cell carcinoma (RCC) cells. An scFv-Fc fusion version of this antibody was successfully used in an internalization study of anti-CA IX scFv-Fc antibody fusion proteins into stable G250-293T cells, exhibiting mild (~20%) inhibition (US8466263B2). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |