Mfg Part Number
Ab04819-1.1
Vector Labs Ab04819-1.1 Anti-25-hydroxyvitamin D [AbAb2-VD], Mouse IgG1, kappa (100 μg)
Quick Overview
The original antibody was generated by subcutaneously immunizing New Zealand rabbits with a conjugate of 25-OH-VD and BSA emulsified with an equal amount of Freund's complete adjuvant.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | AbAb2-VD |
| Synonyms: | 25-OH-VD; 25(OH)D; vitamin D; calciferol |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; colloidal gold immunochromatography |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using the variable domain sequences of the original Rabbit IgG format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody binds 25-hydroxyvitamin D. Vitamin D (calciferol) is an essential fat-soluble vitamin responsible for absorption and regulation of calcium and phosphorus, maintaining bone health, regulating immune function, preventing cardiovascular disease, and regulating fat and energy metabolism. It is important for the typical growth and development of bones and teeth and for improving resistance to certain diseases. Deficiency of vitamin may lead to osteoporosis or rickets. |
| Application Notes: | This antibody binds 25-hydroxyvitamin D in an ELISA with an EC50 of 4.72 ng/mL and the IgG version of the antibody as a binding affinity in the sub-picomolar range. This antibody in combination with AbAb1-VD can be used in the development of double-antibody sandwich colloidal gold immunochromatography test strips, where AbAb1-VD is used to prepare Au-antibody complex and AbAb2-VD is used for T line coating. |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |