JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript in your browser.

Skip to the beginning of the images gallery

Mfg Part Number

Ab04869-1.1

Vector Labs Ab04869-1.1 Anti-H3K9ac [scFv-58F], Mouse IgG1, kappa (100 μg)

VECTOR LABS

Be the first to review this product

Quick Overview

The original antibody was isolated from a mouse naïve ScFv library by panning against the acetylated lysine 9 of Histone H3.

$570.70

Order will ship within 1-3 business days

Description

Product Class: Purified
Clone ID: scFv-58F
Synonyms: acetylated lysine 9 of histone H3
Amount: 100 μg
Application Codes Clone: ELISA; WB; IP; IF
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: scFv
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This full-length, reformatted mouse antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: The original antibody is strongly specificity for the acetylated lysine 9 of Histone H3. It does not cross-react with any other acetylated, phosphorylated, or methylated residue of histone H3, nor with any other histone protein.
Application Notes: The specificity of the original format of the antibody for acetylated lysine 9 of histone H3 was confirmed by ELISA analysis. The original format of the antibody detected acetylated lysine 9 of histone H3 by western blot analysis. The original format of the antibody could immunoprecipitate recombinant native acetylated histone H3 in vitro. The original format of the antibody-bound yeast acetylated H3 in vivo, as it was able to immunoprecipitate it after cell lysis and chromatin sonication. Moreover, the intracellular expressing antibody was able to reduce acetylated histone availability in chromatin. Immunofluorescence was performed on HeLa cells using this antibody. The antibody strongly decorates nuclei of HeLa cells, with the exclusion of perinuclear (lamin positive) regions, indicative of euchromatic staining (US11137393B2).

Product Class:	Purified
Clone ID:	scFv-58F
Synonyms:	acetylated lysine 9 of histone H3
Amount:	100 μg
Application Codes Clone:	ELISA; WB; IP; IF
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	scFv
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This full-length, reformatted mouse antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	The original antibody is strongly specificity for the acetylated lysine 9 of Histone H3. It does not cross-react with any other acetylated, phosphorylated, or methylated residue of histone H3, nor with any other histone protein.
Application Notes:	The specificity of the original format of the antibody for acetylated lysine 9 of histone H3 was confirmed by ELISA analysis. The original format of the antibody detected acetylated lysine 9 of histone H3 by western blot analysis. The original format of the antibody could immunoprecipitate recombinant native acetylated histone H3 in vitro. The original format of the antibody-bound yeast acetylated H3 in vivo, as it was able to immunoprecipitate it after cell lysis and chromatin sonication. Moreover, the intracellular expressing antibody was able to reduce acetylated histone availability in chromatin. Immunofluorescence was performed on HeLa cells using this antibody. The antibody strongly decorates nuclei of HeLa cells, with the exclusion of perinuclear (lamin positive) regions, indicative of euchromatic staining (US11137393B2).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

Reviews