Mfg Part Number
Ab04887-3.0
Vector Labs Ab04887-3.0 Anti-CD20 [HB20-3], Mouse IgG2b, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing BALB/c mice with a mouse pre-B cell line that was transfected with cDNAs encoding human CD20.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | HB20-3 |
| Synonyms: | Bp35; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1 |
| Amount: | 100 μg |
| Application Codes Clone: | IF; Depletion |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Specificity Statement: | The original antibody is specific for CD20. |
| Application Notes: | The original format of the antibody bound to cell surface CD20, such as human blood lymphocytes and the Raji B lymphoblastoid cell line, by immunofluorescence analysis. The original format of the antibody bound to primary B cells and B lymphoma cell lines expressing CD20 in indirect immunofluorescence staining assays (US9416187B2). The IgG1 and IgG1 YTE formats of the antibody were able to efficiently elicit ADCC with an IC50 of 1.3 ± 0.5 and 3.7 ± 1.4 ng/mL, respectively with Daudi as target cells and 4.6 ± 1.6 and 5.2 ± 1.7 ng/mL with Raji cells. Further, the IgG1 and IgG1 YTE formats of the antibody were able to potently elicit CDC with IC50 values of 34 and 160 ng/mL, respectively (Borrok et al., 2017; PMID: 28057542). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |