Mfg Part Number
Ab05001-23.0
Vector Labs Ab05001-23.0 Anti-H3K9me3 [2D2-A1], Rabbit IgG, kappa (100 μg)
Quick Overview
The original antibody was generated by panning a page display library against the 4-5 Fab in complex with the Kme3-containing histone peptides.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | 2D2-A1 |
| Synonyms: | H3K9; trimethyl-Histone H3 (Lys9); histone H3; Trimethylated Histone H3; Trimethylated Lys; Trimethylated Lys9; Histone H3 trimethylated at Lysine 9; post translational modification; histone post-translational modification |
| Amount: | 100 μg |
| Application Codes Clone: | IP |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | The antibody binds H3K9me3. |
| Application Notes: | The specificity of the original format of the antibody was confirmed by yeast surface display assay (Kd= 0.9 nM). The antibody, together with 4-5 was employed to build an heterodimeric clasping antibody with enhanced binding properties. The scFv format of the antibody, together with 4-5 was selective for H3K9me3 in an IP assay (Kd= 0.4 nM) (Riso et al., 2025 ; PMID: 39793060). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |