Vector Labs Ab05019-10.0 Anti-DNP [SPE7], Human IgG1, lambda (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: SPE7
Synonyms: 2,4-DNP; 2,4-dinitrophenol; C6H4N2O5; C6H3(OH)(NO2)2; Aldifen; Phenol, 2,4-dinitro-; Nitrophen; CHEBI:42017; CAS:51-28-5
Amount: 100 μg
Application Codes Clone: ELISA
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgE
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgE format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds 2,4 dinitrophenol (DNP), also binds related DNP analogs like 2-nitrophenol (NP) and 2-nitro-4-iodophenol (PMID: 12610298) and some unrelated compounds like alizarin red (Az, Kd=40 nM) and furazolidone (Fur, Kd= 1.2M) (PMID: 1650428). Anti-DNP antibodies can be used as an independent quality control system in immunochromatography which can improve the specificity of C-line. They also have applications in visualization of DNP-labeled nucleic acid probes in techniques like in situ hybridization (ISH) and immunohistochemistry (IHC). They also serve as isotype controls in flow cytometry, immunofluorescence, and other assays to validate specificity.
Application Notes: This antibody is one of the most widely used mouse IgE to study IgE biology and function. The original mouse IgE antibody binds DNP with an affinity of 20nM. Its affinity for close analogs of DNP, such as 2-nitrophenol (NP) and 2-nitro-4-iodophenol, is negligible (Kd >100 µM). The binding characterization of this antibody was done using ELISA. SPE-7 is reported to have the ability to trigger mast cell activation and survival independent of a cross-linking antigen (PMID: 28811536; 12360215) the potential to bind DNP-unrelated antigens through conformational diversity (PMID: 12610298) and an increased capability to bind mouse Fcγ receptors (PMID: 17558411).

Product Class:	Purified
Clone ID:	SPE7
Synonyms:	2,4-DNP; 2,4-dinitrophenol; C6H4N2O5; C6H3(OH)(NO2)2; Aldifen; Phenol, 2,4-dinitro-; Nitrophen; CHEBI:42017; CAS:51-28-5
Amount:	100 μg
Application Codes Clone:	ELISA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgE
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgE format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds 2,4 dinitrophenol (DNP), also binds related DNP analogs like 2-nitrophenol (NP) and 2-nitro-4-iodophenol (PMID: 12610298) and some unrelated compounds like alizarin red (Az, Kd=40 nM) and furazolidone (Fur, Kd= 1.2M) (PMID: 1650428). Anti-DNP antibodies can be used as an independent quality control system in immunochromatography which can improve the specificity of C-line. They also have applications in visualization of DNP-labeled nucleic acid probes in techniques like in situ hybridization (ISH) and immunohistochemistry (IHC). They also serve as isotype controls in flow cytometry, immunofluorescence, and other assays to validate specificity.
Application Notes:	This antibody is one of the most widely used mouse IgE to study IgE biology and function. The original mouse IgE antibody binds DNP with an affinity of 20nM. Its affinity for close analogs of DNP, such as 2-nitrophenol (NP) and 2-nitro-4-iodophenol, is negligible (Kd >100 µM). The binding characterization of this antibody was done using ELISA. SPE-7 is reported to have the ability to trigger mast cell activation and survival independent of a cross-linking antigen (PMID: 28811536; 12360215) the potential to bind DNP-unrelated antigens through conformational diversity (PMID: 12610298) and an increased capability to bind mouse Fcγ receptors (PMID: 17558411).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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