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Mfg Part Number

Ab05023-23.0

Vector Labs Ab05023-23.0 Anti-Clostridium difficile toxin A [CA997], Rabbit IgG, kappa (100 μg)

VECTOR LABS

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Quick Overview

The antibody was generated by immunizing Sprague Dawley rats and half-lop rabbits with various antigens, including synthetic peptides mapping to regions common to both TcdA and TcdB full-length toxins, formaldehyde-inactivated toxoid A, binding domain fragments of Toxin A, binding domain fragments of Toxin B, or combinations of these. Following immunization, B cells were isolated, and the resulting antibodies were subsequently humanized.

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Description

Product Class: Purified
Clone ID: CA997
Synonyms: CA164_00997; CA164_997; CA164_0997; tcdA; toxA; Toxin A; Glucosyltransferase TcdA
Amount: 100 μg
Application Codes Clone: in vitro; in vivo; SPR
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Human format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for Toxin A from Clostridium difficile.
Application Notes: This antibody was used for in vitro neutralization assay on CACO-2 cells with toxin A, it was shown that this antibody has a high potency as neutralization agent, it had a substantial potential to neutralize TEER loss with maximal inhibition approaching 80% and an EC50 of approximately 80 ng/ml. This antibody was used for surface plasmon resonance on the subdomains of TcdA123 and Tcd456, it showed it was able to bind at least 12 times to Toxin A with an affinity of 132 pM. This antibody was used for an in vivo hamster infection study, it was able to improve survivability (Davies et al., 2013; PMID:23324518).

Product Class:	Purified
Clone ID:	CA997
Synonyms:	CA164_00997; CA164_997; CA164_0997; tcdA; toxA; Toxin A; Glucosyltransferase TcdA
Amount:	100 μg
Application Codes Clone:	in vitro; in vivo; SPR
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric rabbit antibody was made using the variable domain sequences of the original Human format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for Toxin A from Clostridium difficile.
Application Notes:	This antibody was used for in vitro neutralization assay on CACO-2 cells with toxin A, it was shown that this antibody has a high potency as neutralization agent, it had a substantial potential to neutralize TEER loss with maximal inhibition approaching 80% and an EC50 of approximately 80 ng/ml. This antibody was used for surface plasmon resonance on the subdomains of TcdA123 and Tcd456, it showed it was able to bind at least 12 times to Toxin A with an affinity of 132 pM. This antibody was used for an in vivo hamster infection study, it was able to improve survivability (Davies et al., 2013; PMID:23324518).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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