Mfg Part Number
Ab05037-3.0
Vector Labs Ab05037-3.0 Anti-Tryptase [E104.v2], Mouse IgG2b, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing two rabbits with CHO-derived human tryptase beta 1 (tetramer) with Freund's complete adjuvant. The humanized version huE104.v2 was generated by grafting the CDRs of the rabbit antibody onto human framework regions.
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| Product Class: | Purified |
|---|---|
| Clone ID: | E104.v2 |
| Synonyms: | TPSAB1; TPS1; TPS2; TPSB1; Tryptase-1; Tryptase alpha/beta-1; Tryptase I; Tryptase alpha-1; Beta tryptase; β-type tryptase; β tryptase; huE104.v2 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody binds human and cynomolgus monkey tryptase, which is a major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Elevated tryptase levels are found in subgroups of patients with mastocytosis, myelodysplastic syndrome, myeloproliferative neoplasm, acute myeloid leukemia, chronic myeloid leukemia (CML) and chronic eosinophilic leukemia (CEL). It could therefore serve to have diagnostic or prognostic significance. |
| Application Notes: | The binding of this antibody to DNP and other related haptes was checked using ELISA. AN02 and related antibodies were engineered for nuclear magnetic resonance (NMR) to enable sequence-specific resonance assignments, aiding in structural analysis of antibody-antigen interactions (PMID: 3375235). The crystal structure of AN02 Fab fragment complexed with DNP-hapten was resolved at 2.9 Å resolution using X-ray crystallography. It was reported that the DNP ring was sandwiched between tryptophan residues Trp96 of the heavy chain and Trp91 of the light chain (PMID: 1920408). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |