Mfg Part Number
Ab05050-10.3
Vector Labs Ab05050-10.3 Anti-C3D [3d8b], Human IgG1, Fc Silent™, kappa (100 μg)
Quick Overview
This antibody was raised by immunizing a mouse with a targeted deletion of the C3 gene with human C3d.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | 3d8b |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | C3; CPAMD1; Complement C3; Complement C3d fragment; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; SPR; WB; in vivo; in vitro; IF |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2b |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This is a reformatted human IgG1 Fc Silent Fc Silent™ antibody, based on the original human IgG2b format, created for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the C-terminus of C3d the epitope is within amino acids 295-309. |
| Application Notes: | This antibody was used for an ELISA on human C3d. This antibody was used for a western blot on human C3d, it showed strong binding. The affinity of this antibody to C3d was determined using surface plasmon resonance, it showed strong binding with a Kds of 0.47 nM. This antibody also showed it could inhibit the CR2-C3d interaction in a competition ELISA. This antibody was used in vivo on mice, it was shown that this antibody could detect tissue- bound C3 fragments, it readily detected C3 along the glomerular basement membrane (Thurman et al., 2013; PMID:23619360). The structure of this antibody was determined using x-ray crystallography. A fusion protein of this antibody with the first 10 consensus repeats of the human CR1 protein was used in vitro for a Wieslab assay, a hemolysis assay and a HMEC assay. The pharmacokinetics (PK) in mice were determined for various fusion proteins containing this antibody (Fahnoe et al., 2022; PMID:35784298). A fusion protein of this antibody with factor H was used for a complement inhibition assay it showed an IC50 of 73 nM. This antibody was used for immunofluorescence on human skin biopsies and kidney tissues (Liu et al., 2024; PMID:38382529). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |