Vector Labs Ab05080-10.3 Anti-Aquaporin 4 [rAb-53 (affinity matured)], Human IgG1, Fc Silent™, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: rAb-53 (affinity matured)
Heavy Chain Modification: Fc Silent™
Synonyms: AQP4; AQP-4; AQmab; aquaporumab; Aquaporin-4; MIWC; WCH4; Mercurial-insensitive water channel; rAb53; Aquaporumab; AQmabAM
Amount: 100 μg
Application Codes Clone: functional assay; cell-based ELISA; IF
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds an extracellular conformation specific epitope on the human aquaporin 4 (AQP4), a water channel expressed on astrocytes throughout the CNS and in skeletal muscle and some epithelial cells outside of the CNS. It plays an important role in brain water homeostasis and in glymphatic solute transport. Neuromyelitis optica spectrum disorder (NMOSD) is an inflammatory disease of the central nervous system (CNS) that is associated with demyelination and neurological deficits in optic nerve, spinal cord and brainstem. Most NMOSD patients are seropositive for IgG autoantibodies ( NMO-IgG) directed against extracellular epitopes of aquaporin-4 (AQP4). Prevention of the binding of NMO-IgG, therefore, may alleviate the disease.
Application Notes: This antibody is an affinity matured version of rAb-53 (Aquaporumab). The binding of this antibody to the extracellular region of AQP4 in CHO cells stably expressing the M23 isoform of human AQP4 was done using a cell-based ELISA. When AQP4-expressing cells were incubated with affinity matured rAb-53 and human complement antibody it produced complement-dependent cytotoxicity (CDC) with EC50 ~ 82 ng/ml. The Fc silent version of this antibody with LALA mutations, also called as AQmabAM bound to AQP4 in cell cultures with Kd ~ 18 ng/ml (~ 0.12 nM). AQmabAM prevented CDC produced by sera from eight NMO patients with IC50 ranging from 40–80 ng/ml, and similarly prevented antibody-dependent cellular cytotoxicity (ADCC). Mechanistic studies demonstrated that AQmabAM blocked binding of serum NMO autoantibodies to AQP4. This antibody can be used for the visualization of AQP4 on the surface of cells using immunofluorescence (PMID: 31654702).

Product Class:	Purified
Clone ID:	rAb-53 (affinity matured)
Heavy Chain Modification:	Fc Silent™
Synonyms:	AQP4; AQP-4; AQmab; aquaporumab; Aquaporin-4; MIWC; WCH4; Mercurial-insensitive water channel; rAb53; Aquaporumab; AQmabAM
Amount:	100 μg
Application Codes Clone:	functional assay; cell-based ELISA; IF
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This is a reformatted human IgG1 Fc Silent Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds an extracellular conformation specific epitope on the human aquaporin 4 (AQP4), a water channel expressed on astrocytes throughout the CNS and in skeletal muscle and some epithelial cells outside of the CNS. It plays an important role in brain water homeostasis and in glymphatic solute transport. Neuromyelitis optica spectrum disorder (NMOSD) is an inflammatory disease of the central nervous system (CNS) that is associated with demyelination and neurological deficits in optic nerve, spinal cord and brainstem. Most NMOSD patients are seropositive for IgG autoantibodies ( NMO-IgG) directed against extracellular epitopes of aquaporin-4 (AQP4). Prevention of the binding of NMO-IgG, therefore, may alleviate the disease.
Application Notes:	This antibody is an affinity matured version of rAb-53 (Aquaporumab). The binding of this antibody to the extracellular region of AQP4 in CHO cells stably expressing the M23 isoform of human AQP4 was done using a cell-based ELISA. When AQP4-expressing cells were incubated with affinity matured rAb-53 and human complement antibody it produced complement-dependent cytotoxicity (CDC) with EC50 ~ 82 ng/ml. The Fc silent version of this antibody with LALA mutations, also called as AQmabAM bound to AQP4 in cell cultures with Kd ~ 18 ng/ml (~ 0.12 nM). AQmabAM prevented CDC produced by sera from eight NMO patients with IC50 ranging from 40–80 ng/ml, and similarly prevented antibody-dependent cellular cytotoxicity (ADCC). Mechanistic studies demonstrated that AQmabAM blocked binding of serum NMO autoantibodies to AQP4. This antibody can be used for the visualization of AQP4 on the surface of cells using immunofluorescence (PMID: 31654702).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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