Vector Labs Ab05081-10.3 Anti-Aquaporin 4 [rAb58], Human IgG1, Fc Silent™, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: rAb58
Heavy Chain Modification: Fc Silent™
Synonyms: AQP4; AQP-4; Aquaporin-4; MIWC; WCH4; Mercurial-insensitive water channel
Amount: 100 μg
Application Codes Clone: FC; IF; functional assay
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This is a reformatted human IgG1 Fc Silent Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds an extracellular conformation specific epitope on the human aquaporin 4 (AQP4). It is capable of binding both M1 and M23 isoforms of AQP4. Aquaporin 4 is a water channel expressed on astrocytes throughout the CNS and in skeletal muscle and some epithelial cells outside of the CNS. It plays an important role in brain water homeostasis and in glymphatic solute transport. Neuromyelitis optica spectrum disorder (NMOSD) is an inflammatory disease of the central nervous system (CNS) that is associated with demyelination and neurological deficits in optic nerve, spinal cord and brainstem. Most NMOSD patients are seropositive for IgG autoantibodies (NMO-IgG) directed against extracellular epitopes of aquaporin-4 (AQP4). Prevention of the binding of NMO-IgG, therefore, may alleviate the disease.
Application Notes: This antibody was reported to mediate AQP4-directed antibody-dependent cellular cytotoxicity and complement-mediated lysis in vitro. The binding characterization of this antibody to AQP4 expressed on Human Fetal Astrocyte (HFA) was done by quantitative flow cytometry (PMID: 19938104). Binding of rAb-58 to the M1 and M23 AQP4 isoforms was measured by the fluorescence ratio imaging method (PMID: 21454592). CHO cells expressing M1- or M23-AQP4 when labeled with rAb-58 conjugated to the red fluorophore Cy3 (rAb-58-Cy3), showed rapid internalization of M23-AQP4 than M1-AQP4 when expressed separately in transfected cells. When both isoforms were co-expressed in OAPs in transfected cells, the antibody causes simultaneous cointernalization of M1- and M23-AQP4 isoforms (PMID: 22987455).

Product Class:	Purified
Clone ID:	rAb58
Heavy Chain Modification:	Fc Silent™
Synonyms:	AQP4; AQP-4; Aquaporin-4; MIWC; WCH4; Mercurial-insensitive water channel
Amount:	100 μg
Application Codes Clone:	FC; IF; functional assay
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This is a reformatted human IgG1 Fc Silent Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds an extracellular conformation specific epitope on the human aquaporin 4 (AQP4). It is capable of binding both M1 and M23 isoforms of AQP4. Aquaporin 4 is a water channel expressed on astrocytes throughout the CNS and in skeletal muscle and some epithelial cells outside of the CNS. It plays an important role in brain water homeostasis and in glymphatic solute transport. Neuromyelitis optica spectrum disorder (NMOSD) is an inflammatory disease of the central nervous system (CNS) that is associated with demyelination and neurological deficits in optic nerve, spinal cord and brainstem. Most NMOSD patients are seropositive for IgG autoantibodies (NMO-IgG) directed against extracellular epitopes of aquaporin-4 (AQP4). Prevention of the binding of NMO-IgG, therefore, may alleviate the disease.
Application Notes:	This antibody was reported to mediate AQP4-directed antibody-dependent cellular cytotoxicity and complement-mediated lysis in vitro. The binding characterization of this antibody to AQP4 expressed on Human Fetal Astrocyte (HFA) was done by quantitative flow cytometry (PMID: 19938104). Binding of rAb-58 to the M1 and M23 AQP4 isoforms was measured by the fluorescence ratio imaging method (PMID: 21454592). CHO cells expressing M1- or M23-AQP4 when labeled with rAb-58 conjugated to the red fluorophore Cy3 (rAb-58-Cy3), showed rapid internalization of M23-AQP4 than M1-AQP4 when expressed separately in transfected cells. When both isoforms were co-expressed in OAPs in transfected cells, the antibody causes simultaneous cointernalization of M1- and M23-AQP4 isoforms (PMID: 22987455).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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