Mfg Part Number
Ab05088-10.0
Vector Labs Ab05088-10.0 Anti-G Protein [CR57], Human IgG1, lambda (100 μg)
Quick Overview
The original antibody was isolated from a scFv phage display library constructed using peripheral blood lymphocytes of rabies-vaccinated donors by panning against rabies virus glycoprotein.
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| Product Class: | Purified |
|---|---|
| Clone ID: | CR57 |
| Synonyms: | CR-57 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; FC; Neutralizing; SPR |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | scFv |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This full-length, reformatted human antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | The antibody is specific for the G Protein of rabies virus. |
| Application Notes: | The specificity of the original format of the antibody was confirmed by competitive ELISA. The original format of the antibody was used in a competition experiments on rabies virus G protein (ERA strain) transfected PER.C6 cells by means of flow cytometry. The signal obtained with the biotinylated antibody was not reduced significantly with the negative control antibody. Competition with the unlabeled antibody reduced the signal to background levels. The binding affinity of the scFv fragments to purified rabies glycoprotein was measured by surface plasmon resonance (Kd= 2.4 nM). The neutralizing potency of the original format of the antibody to rabies G protein IgGs was determined using an in vitro neutralization assay (Modified RFFIT). The antibody could neutralize anti-rabies virus G protein with a potencies of 3800 IU/mg (US7579446B2). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |