Mfg Part Number
Ab05092-10.0
Vector Labs Ab05092-10.0 Anti-Envelope protein [E3], Human IgG1, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing chimpanzees with inactivated JE-VAX and then boosted with attenuated JEV SA14-14-2, followed by phage library construction. The antibody was isolated by panning against domain III-specific E.
$570.70
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | E3 |
| Synonyms: | NS1'; Core protein; Protein prM; Peptide pr; Structural polyprotein; Capsid protein C; Small envelope protein M; Matrix protein; Envelope protein E; Non-structural protein 1' |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; SPR; neutralizing |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | Fab |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Rhesus Macaque Fab format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | The antibody is specific for Lys179 (domain I) in the envelope protein. |
| Application Notes: | The specificity of the antibody was confirmed by ELISA analysis (Kd= 0.98 nM). The binding affinity of the Fab fragments to recombinant JEV E protein was measured by surface plasmon resonance (Kd = 0.35 nM). The antibody could neutralize wild-type JEV by competition ELISA (Goncalvez et al., 2008; PMID: 18480437). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |