Mfg Part Number
Ab05093-10.0
Vector Labs Ab05093-10.0 Anti-huntingtin protein [MW7], Human IgG1, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing mice with Htt exon 1 (67Q) protein fused to GST (GST-HDx67Q). Boosting of the mice was performed with an aggregated form of exon 1 having 67 Q repeats of the huntingtin protein (67Q), prepared by removing the GST.
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| Product Class: | Purified |
|---|---|
| Clone ID: | MW7 |
| Synonyms: | Htt; HD protein; Huntingtin; Huntington disease protein; Milton Wexler 7 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; WB; IHC; IF |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgM |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | The antibody is specific for huntingtin protein. In particular, it specifically binds peptides that contain the polyP domain in Htt. |
| Application Notes: | The specificity of the antibody was confirmed by ELISA analysis. The antibody detected the huntingtin protein in 293 cells by western blot analysis. The original format of the antibody bound the expanded repeat mutant form of Htt in mouse brain extracts. In human lymphoblastoma cell extracts, the original format of the antibody bound the high molecular weight Htt very weakly, but binds strongly one or two smaller molecular weight proteins which are present in normal (HD7) and Huntington's disease (HD2) human lymphoblastoma cell extracts. The original format of the antibody displayed punctate perinuclear or nuclear membrane staining in wild-type and mutant brain sections with weaker punctate perinuclear or nuclear membrane staining, but more prominent nuclear inclusion staining of mice expressing a human chimeric Htt exon1 construct with 94Q repeats. The scFv format of the antibody inhibited the toxicity of mutant Htt expressed on 293 cells. The effect of scFv format of the antibody expression on mutant Htt aggregation in 293 cells was evaluated, very little aggregated Htt was found in the presence of the antibody (US7375194B2). The antibody could differentiate transgenic from WT mice by staining nuclear inclusions in R6/2 brain (Ko et al., 2001; PMID: 11719267). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |