Mfg Part Number
Ab05099-10.3
Vector Labs Ab05099-10.3 Anti-uPAR [2.6.1], Human IgG1, Fc Silent™, kappa (100 μg)
Quick Overview
The original antibody was generated by immunizing XenoMouse® with human uPAR expressed on the surface of B cells. Recovered lymphocytes from hyperimmunized mice are fused with a myeloid-type cell line to prepare immortal hybridoma cell lines. Antibodies from the cell supernatants are then screened by ELISA for reactivity against the uPAR immunogen.
$570.70
Order will ship within 1-3 business days
| Product Class: | Purified |
|---|---|
| Clone ID: | 2.6.1 |
| Heavy Chain Modification: | Fc Silent™ |
| Synonyms: | CD87; U-PAR; MO3; PLAUR; Urokinase plasminogen activator surface receptor; Monocyte activation antigen Mo3 |
| Amount: | 100 μg |
| Application Codes Clone: | inhibit; functional assay; FC |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG2 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This is a reformatted human IgG1 Fc Silent Fc Silent™ antibody, based on the original human IgG2 format, created for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody binds human urokinase plasminogen activator receptor (uPAR). It also cross reacts with cynomolgus monkey UPAR. It plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. Upregulation of uPAR expression at the cell surface, and plasminogen activation, has been linked to a number of conditions including inflammation, wound repair, arthritis, atherosclerosis angiogenesis, tumor invasion and metastasis. |
| Application Notes: | This antibody inhibits uPA binding to the cell surface of U937 cells. In an adhesion assay, this antibody inhibited cell adhesion of U937 cells to vitronectin in a dose-dependent manner. At a concentration of 2 μg/ml, 2.6.1 achieved 90% of inhibition. The binding characterization of this antibody to human UPAR was done using High Resolution Biacore™. The binding affinity of the original IgG isotype for human UPAR was reported to be Kd= 249pM. The binding characterization of biotin conjugated version of this antibody was done using immunohistochemistry on frozen cell pellets and normal human tissue microarrays (US8026344). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |