Vector Labs Ab05101-10.0 Anti-Granzyme-B [Fv17], Human IgG1, lambda (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: Fv17
Synonyms: GZMB; C11; CTLA-1; CTLA1; CTSGL1; CGL1; CSPB; GRB; HLP; SECT; Granzyme-2; Cathepsin G-like 1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Human lymphocyte protein; T-cell serine protease 1-3E
Amount: 100 μg
Application Codes Clone: IP; FC; ELISA
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: scfv
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This full-length, reformatted human antibody was made using the variable domain sequences of the original Human scfv format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds granzyme B, an abundant serine protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It has has been demonstrated to be a pivotal molecule for protective immunity against viral infection and cellular malignant transformation.
Application Notes: This antibody can detect the native for of granzyme B and fails to detect its denatured form. This antibody can be used as a tool to monitor granzyme B expression in natural killer cells and antigen specific CD8 T cell immunity by intracellular staining using FITC-labelled Fv17 together with PE-labelled anti-perforin IgG. Stained cells were analyzed by flow cytometry. This antibody failed to detect granzyme B immobilized on plastic in a direct ELISA. This antibody in combination with other commercially available anti-granzyme B antibody BG10 can be used for sandwich ELISA. This antibody can also be used to detect granzyme B and granzyme B-PI9 conjugate using immunoprecipitation (PMID: 15319069).

Product Class:	Purified
Clone ID:	Fv17
Synonyms:	GZMB; C11; CTLA-1; CTLA1; CTSGL1; CGL1; CSPB; GRB; HLP; SECT; Granzyme-2; Cathepsin G-like 1; Cytotoxic T-lymphocyte proteinase 2; Lymphocyte protease; Fragmentin-2; Human lymphocyte protein; T-cell serine protease 1-3E
Amount:	100 μg
Application Codes Clone:	IP; FC; ELISA
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	scfv
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This full-length, reformatted human antibody was made using the variable domain sequences of the original Human scfv format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds granzyme B, an abundant serine protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It has has been demonstrated to be a pivotal molecule for protective immunity against viral infection and cellular malignant transformation.
Application Notes:	This antibody can detect the native for of granzyme B and fails to detect its denatured form. This antibody can be used as a tool to monitor granzyme B expression in natural killer cells and antigen specific CD8 T cell immunity by intracellular staining using FITC-labelled Fv17 together with PE-labelled anti-perforin IgG. Stained cells were analyzed by flow cytometry. This antibody failed to detect granzyme B immobilized on plastic in a direct ELISA. This antibody in combination with other commercially available anti-granzyme B antibody BG10 can be used for sandwich ELISA. This antibody can also be used to detect granzyme B and granzyme B-PI9 conjugate using immunoprecipitation (PMID: 15319069).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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