Mfg Part Number
Ab05116-23.0
Vector Labs Ab05116-23.0 Anti-LASV glycoprotein complex [M30], Rabbit IgG, kappa (100 μg)
Quick Overview
The original antibody was generated by introducing two mutations (R54 insertion and L100R) into the sequence of the parent clone 18.5C (an isolate from a human survivor of Lassa fever).
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| Product Class: | Purified |
|---|---|
| Clone ID: | M30 |
| Synonyms: | Lassa virus glycoprotein complex; GPC; Pre-glycoprotein polyprotein GP complex; Pre-GP-C; Stable signal peptide; SSP; Glycoprotein G1; GP1; Glycoprotein G2; GP2; 18.5C/R59ins-L108R; 18.5C-M30 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; Neutralization; in vitro; CryoEM |
| Shipping Temperature: | Wet Ice |
| Origin Pub PMID: | 35730904 |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for the LASV glycoprotein complex (GPC). It binds to a pre-fusion epitope of the LASV GPC. |
| Application Notes: | The original antibody (human IgG1) was generated and shown to completely neutralize 100% of rVSV-LASV-LI particles with an IC50 of 2–4 nM. It also completely neutralized authentic LASV-LI in a Plaque-Reduction Neutralization Test (PRNT) (Hastie et al., 2019; PMID: 31398326). BLI characterization revealed high binding affinity, with apparent KD values of 0.559 nM, 0.494 nM, 0.154 nM, and 47.0 pM for LASV lineage I (LI) GP, LI-R198S-GP, LI-Q397H-GP, and LASV lineage IV (LIV) GP, respectively (Buck et al., 2022; PMID: 35730904). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |