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Mfg Part Number

Ab05118-21.0

Vector Labs Ab05118-21.0 Anti-Capsular polysaccharide [2-2-B (5C1-3H7)], Mouse IgM, kappa (50 μg)

VECTOR LABS

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Quick Overview

The original antibody was generated by immunizing mice with whole encapsulated N. Meningitidis.

$692.90

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Description

Product Class: Purified
Clone ID: 2-2-B (5C1-3H7)
Synonyms: Capsular PS; type B capsular PS; serogroup B sialic acid containing capsular polysaccharides; MBPS
Amount: 50 μg
Application Codes Clone: ELISA; FC; immunogold EM
Shipping Temperature: Wet Ice
Origin Pub PMID: 15589322
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgM
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody is specific for the serogroup B capsular polysaccharides of N. meningitidis.
Application Notes: The original antibody format (Mouse IgM) was generated and evaluated. Its specificity was determined by indirect whole‑cell and purified polysaccharide ELISA. It exhibited strong binding to serogroup B capsular polysaccharide (OD₄₀₅ ≈ 0.85 on strain 99M) with only background‑level reactivity to serogroup C and the other 12 serogroups, demonstrating exquisite type B specificity (Berry et al., 2005; PMID: 15589322). This antibody was successfully used to investigate capsule expression in Neisseria meningitidis serogroup B. It was used in a whole-cell ELISA to quantify total capsular polysaccharide production, in flow cytometry to detect surface and intracellular capsular polysaccharide, and in immunogold electron microscopy to visualize capsular polysaccharide localization (Tzeng et al., 2005; PMID: 15731047). This antibody underwent genetic analysis to reveal its unmutated V genes, structural modeling to identify a shallow combining site groove, and used as a control for autoreactivity to confirm strong PSA binding, contrasting with non-autoreactive anti-N-Pr MBPS mAbs (Moe et al., 2006; PMID: 16140379).

Product Class:	Purified
Clone ID:	2-2-B (5C1-3H7)
Synonyms:	Capsular PS; type B capsular PS; serogroup B sialic acid containing capsular polysaccharides; MBPS
Amount:	50 μg
Application Codes Clone:	ELISA; FC; immunogold EM
Shipping Temperature:	Wet Ice
Origin Pub PMID:	15589322
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgM
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody is specific for the serogroup B capsular polysaccharides of N. meningitidis.
Application Notes:	The original antibody format (Mouse IgM) was generated and evaluated. Its specificity was determined by indirect whole‑cell and purified polysaccharide ELISA. It exhibited strong binding to serogroup B capsular polysaccharide (OD₄₀₅ ≈ 0.85 on strain 99M) with only background‑level reactivity to serogroup C and the other 12 serogroups, demonstrating exquisite type B specificity (Berry et al., 2005; PMID: 15589322). This antibody was successfully used to investigate capsule expression in Neisseria meningitidis serogroup B. It was used in a whole-cell ELISA to quantify total capsular polysaccharide production, in flow cytometry to detect surface and intracellular capsular polysaccharide, and in immunogold electron microscopy to visualize capsular polysaccharide localization (Tzeng et al., 2005; PMID: 15731047). This antibody underwent genetic analysis to reveal its unmutated V genes, structural modeling to identify a shallow combining site groove, and used as a control for autoreactivity to confirm strong PSA binding, contrasting with non-autoreactive anti-N-Pr MBPS mAbs (Moe et al., 2006; PMID: 16140379).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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