Vector Labs Ab05119-1.1 Anti-Capsular polysaccharide [C2/655.7], Mouse IgG1, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: C2/655.7
Synonyms: Capsular PS; meningococcal polysaccharide group C; serogroup C sialic acid containing capsular polysaccharides
Amount: 100 μg
Application Codes Clone: ELISA; FELISA; WB
Shipping Temperature: Wet Ice
Origin Pub PMID: 15589322
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement: This antibody is specific for the α(2→9) sialic‑acid backbone of N. meningitidis's serogroup C capsular polysaccharide. Its acidic pI (5.6–6.7) and shorter IgG1 hinge suggest it approaches the α(2→9) sialic‑acid backbone in a more electrostatically guided, compact orientation than Ab05121 [2016.3], one of our other anti–N. meningitidis group C capsular PS products. Its unique CDR composition also creates a subtly different paratope shape, so although both recognize the same motif, they likely bind from different angles or contact footprints on the polysaccharide chain. Functionally, this antibody shows ~30‑fold higher 50% binding affinity to OAc⁻ and ~10‑fold higher to MCPS.
Application Notes: The original antibody format (Mouse IgG1) was generated and evaluated. Its specificity was determined by FELISA. It exhibited a binding pattern of OAc⁻ >> MCPS, with 50% binding observed at approximately 0.003 µg/mL and 0.3 µg/mL, respectively, under the assay conditions. This indicates a strong preference for the de‑O‑acetylated serogroup C capsular polysaccharide. The original antibody's pI was measured to be in the range of 5.6–6.7 (García-Ojeda et al., 2000; PMID: 10603394). Surface plasmon resonance analysis confirmed its higher avidity and preference for the OAc⁻ surface, although kinetic studies revealed short dissociation half-lives for both OAc+ and OAc⁻ surfaces (García-Ojeda, 2004; PMID: 15155652). This antibody was successfully used in a WB to detect OAc⁻ (Mosley et al., 2009; DOI: 10.3109/10242420903388694).

Product Class:	Purified
Clone ID:	C2/655.7
Synonyms:	Capsular PS; meningococcal polysaccharide group C; serogroup C sialic acid containing capsular polysaccharides
Amount:	100 μg
Application Codes Clone:	ELISA; FELISA; WB
Shipping Temperature:	Wet Ice
Origin Pub PMID:	15589322
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Specificity Statement:	This antibody is specific for the α(2→9) sialic‑acid backbone of N. meningitidis's serogroup C capsular polysaccharide. Its acidic pI (5.6–6.7) and shorter IgG1 hinge suggest it approaches the α(2→9) sialic‑acid backbone in a more electrostatically guided, compact orientation than Ab05121 [2016.3], one of our other anti–N. meningitidis group C capsular PS products. Its unique CDR composition also creates a subtly different paratope shape, so although both recognize the same motif, they likely bind from different angles or contact footprints on the polysaccharide chain. Functionally, this antibody shows ~30‑fold higher 50% binding affinity to OAc⁻ and ~10‑fold higher to MCPS.
Application Notes:	The original antibody format (Mouse IgG1) was generated and evaluated. Its specificity was determined by FELISA. It exhibited a binding pattern of OAc⁻ >> MCPS, with 50% binding observed at approximately 0.003 µg/mL and 0.3 µg/mL, respectively, under the assay conditions. This indicates a strong preference for the de‑O‑acetylated serogroup C capsular polysaccharide. The original antibody's pI was measured to be in the range of 5.6–6.7 (García-Ojeda et al., 2000; PMID: 10603394). Surface plasmon resonance analysis confirmed its higher avidity and preference for the OAc⁻ surface, although kinetic studies revealed short dissociation half-lives for both OAc+ and OAc⁻ surfaces (García-Ojeda, 2004; PMID: 15155652). This antibody was successfully used in a WB to detect OAc⁻ (Mosley et al., 2009; DOI: 10.3109/10242420903388694).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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