Mfg Part Number
Ab05126-10.3

Vector Labs Ab05126-10.3 Anti-Chlamydia lipopolysaccharide [S25-23], Human IgG1, Fc Silent™, kappa (100 μg)

Quick Overview
This antibody was raised by immunizing a BALB/c mouse with KD03-GlcNAc-BSA.
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Product Class:Purified
Clone ID:S25-23
Heavy Chain Modification:Fc Silent™
Synonyms:LPS; Kdo(2→8)Kdo(2→4)Kdo
Amount:100 μg
Application Codes Clone:ELISA; functional assay; WB
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG1
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This is a reformatted human IgG1 Fc Silent Fc Silent™ antibody, based on the original human IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:This antibody is specific for the Kdo(2→8)Kdo(2→4)Kdo from different chlamydia species.
Application Notes:Multiple different formats of this antibody were used for surface plasmon resonance on alpha-Kdo-(2->8)-alpha-Kdo-(2->4)-alpha-Kdo, it showed an affinity between 4.5 * 10^4 M^-1 * s^-1 and 5.9 * 10^4 M^-1 * s^-1 (Müller-Loennies et al., 2000; PMID:10642603). This antibody was used for an ELISA on KDO3-GIcNAc-BSA to characterize this antibody. This antibody was used for a western blot on chlamydia LPS, it showed it was able to bind to r595-207 LPS (Fu et al., 1992; PMID:1372290, Haji-Ghassemi et al., 2018; PMID:30571096, Brade et al., 1997; PMID:9376684). The structure of this antibody was determined using x-ray crystallography (Haji-Ghassemi et al., 2018; PMID:30571096).
More Information
Brand Vector Labs
UNSPSC 12161500