Mfg Part Number
Ab05130-10.0
Vector Labs Ab05130-10.0 Anti-Porphyrin [14H7], Human IgG1, lambda (100 μg)
Quick Overview
This antibody was raised by immunizing a BALB/c mouse with Fe(ToCPP)-KLH.
$570.70
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| Product Class: | Purified |
|---|---|
| Clone ID: | 14H7 |
| Amount: | 100 μg |
| Application Codes Clone: | ELISA; functional assay |
| Shipping Temperature: | Wet Ice |
| Buffer Composition: | PBS with 0.02% Proclin 300. |
| Original Format: | IgG1 |
| Storage Temperature: | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C. |
| Chimeric Use Statement: | This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques. |
| Specificity Statement: | This antibody is specific for porphyrins, with highest sensitivity for iron porphyrins. |
| Application Notes: | The structure of the Fab fragment of this antibody was determined using x-ray crystallography (Robles et al., 2012; PMID:23240001). This antibody was used for an competitive ELISA on porphyrin, it showed that it bound most strongly to (Ortho-COOMePh)4-porphyrin (De Lauzon et al., 1998; PMID:9799110, Quilez et al., 1996; PMID:8849692). This antibody was used for an peroxidase activity experiment, this antibody showed the ability to catalyse the oxidation of 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) by H202 (Quilez et al., 1996; PMID:8849692). |
| Brand | Vector Labs |
|---|---|
| UNSPSC | 12161500 |