Vector Labs Ab05132-10.0 Anti-Factor VIII [BO2C11], Human IgG1, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: BO2C11
Synonyms: AHF; Coagulation factor VIII; Antihemophilic factor; Procoagulant component
Amount: 100 μg
Application Codes Clone: ELISA; WB; SPR; functional assay; in vivo; in vitro
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG4
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This reformatted human antibody was made using the variable domain sequences of the original Human IgG4 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody is specific for amino acids 2125 to 2332 of Factor VIII.
Application Notes: The original antibody was used for an ELISA on factor VIII, in a further ELISA it was also shown that the antibody inhibits factor VIII binding to vWF (Jacquemin et al., 1998; PMID:9657749, Gangadharan et al., 2017; PMID:27758819, Villard et al., 2003; PMID:12676786). This antibody was used for a western blot on reduced factor VIII. This antibody was evaluated in an inhibition assay to assess its ability to suppress plasma factor VIII activity. Using a coagulation assay, it was demonstrated that the antibody reduces factor VIII activity by 50% at a concentration of 0.14 g/mL (Jacquemin et al., 1998; PMID:9657749, Villard et al., 2003; PMID:12676786). In a functional assay it was confirmed that vWF can inhibit the factor VIII inactivation by this antibody, but this effect decreases with incubation time. This antibody was used for surface plasmon resonance on factor VIII (Jacquemin et al., 1998; PMID:9657749). This antibody was used for an in vivo experiment, it was shown that this antibody can cause a 70% reduction in factor VIII internalization by MoDCs. This antibody was used in vitro on dendritic and CD4+ T cells, it was shown that is was able to diminish factor VIII immunogenicity (Gangadharan et al., 2017; PMID:27758819). This antibody was used in vivo on factor VIII deficient mice, they were injected with 0.5 IU in 100 μL physiologic saline of human factor VIII, it was shown that 0.25 ug of this antibody was sufficient to inhibit the factor VIII (Villard et al., 2003; PMID:12676786). The structure of the fab version of this antibody was determined using x-ray crystallography (Spiegel Jr et al., 2001; PMID:11418455).

Product Class:	Purified
Clone ID:	BO2C11
Synonyms:	AHF; Coagulation factor VIII; Antihemophilic factor; Procoagulant component
Amount:	100 μg
Application Codes Clone:	ELISA; WB; SPR; functional assay; in vivo; in vitro
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG4
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This reformatted human antibody was made using the variable domain sequences of the original Human IgG4 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody is specific for amino acids 2125 to 2332 of Factor VIII.
Application Notes:	The original antibody was used for an ELISA on factor VIII, in a further ELISA it was also shown that the antibody inhibits factor VIII binding to vWF (Jacquemin et al., 1998; PMID:9657749, Gangadharan et al., 2017; PMID:27758819, Villard et al., 2003; PMID:12676786). This antibody was used for a western blot on reduced factor VIII. This antibody was evaluated in an inhibition assay to assess its ability to suppress plasma factor VIII activity. Using a coagulation assay, it was demonstrated that the antibody reduces factor VIII activity by 50% at a concentration of 0.14 g/mL (Jacquemin et al., 1998; PMID:9657749, Villard et al., 2003; PMID:12676786). In a functional assay it was confirmed that vWF can inhibit the factor VIII inactivation by this antibody, but this effect decreases with incubation time. This antibody was used for surface plasmon resonance on factor VIII (Jacquemin et al., 1998; PMID:9657749). This antibody was used for an in vivo experiment, it was shown that this antibody can cause a 70% reduction in factor VIII internalization by MoDCs. This antibody was used in vitro on dendritic and CD4+ T cells, it was shown that is was able to diminish factor VIII immunogenicity (Gangadharan et al., 2017; PMID:27758819). This antibody was used in vivo on factor VIII deficient mice, they were injected with 0.5 IU in 100 μL physiologic saline of human factor VIII, it was shown that 0.25 ug of this antibody was sufficient to inhibit the factor VIII (Villard et al., 2003; PMID:12676786). The structure of the fab version of this antibody was determined using x-ray crystallography (Spiegel Jr et al., 2001; PMID:11418455).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

Reviews