Mfg Part Number
Ab05133-10.3

Vector Labs Ab05133-10.3 Anti-Factor VIII [NB2E9], Human IgG1, Fc Silent™, kappa (100 μg)

Quick Overview
This antibody was raised from PMBCs from a hemophilia A patient with inhibitor LE.
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Product Class:Purified
Clone ID:NB2E9
Heavy Chain Modification:Fc Silent™
Synonyms:AHF; Coagulation factor VIII; Antihemophilic factor; Procoagulant component
Amount:100 μg
Application Codes Clone:ELISA; functional assay
Shipping Temperature:Wet Ice
Buffer Composition:PBS with 0.02% Proclin 300.
Original Format:IgG4
Storage Temperature:Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:This is a reformatted human IgG1 Fc Silent Fc Silent™ antibody, based on the original human IgG4 format, created for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:This antibody is specific for amino acids E2066 and R2150 of human Factor VIII.
Application Notes:This antibody was used for an ELISA on recombinant human factor VIII, in a further ELISA is was shown that this antibody could inhibit factor VIII binding to vWF, it had a IC50 of 0.25 ug/mL. It was shown that this antibody could inhibit the function of factor VIII up to 85% (Jacquemin et al., 2000; PMID:10607698, Coxon et al., 2021; PMID:33973229).
More Information
Brand Vector Labs
UNSPSC 12161500