Vector Labs Ab05148-10.0 Anti-VHH antibody [2A3], Human IgG1, kappa (100 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 2A3
Synonyms: VHH; Nb; sdAbs; sdAb; Nanobody; Single-domain antibodies; F233-2A3
Amount: 100 μg
Application Codes Clone: ELISA; IHC; labelling; purification
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement: This antibody binds distinct epitope in the conserved framework regions of llama-derived VHHs and is reactive with 98% of VHH proteins regardless of their antigenic specificity, with no reactivity to human or mouse IgG and reduced reactivity to conventional llama or alpaca IgG. This antibody fails to react with other isotypes and subtypes of human immunoglobulins like human lgG1 , lgG2, lgG3, lgG4, IgA, slgA and IgM.
Application Notes: The binding reactivity of this antibody to various sdAbs was determined using ELISA. The binding of this antibody to the VHH did not disrupt VHH/antigen interaction, and so it is appropriate for secondary labeling to assess cellular or tissue reactivity of VHH molecules. The binding ability of this antibody to detect surface expressed sdAb-based CAR constructs was also tested. This antibody could detect Jurkat T cells stably expressing sdCD22 CAR constructs. It was reported that a 1 :1 mix of antibodies 2A3 and 3H12 provides the best overall breadth of binding to diverse VHH proteins. This antibody was also used for the labelling and staining of frozen human tissues using immunohistochemistry. The tissues were first stained with CD22 specific nanobody and the anti-VHH antibody 2A3 was used a secondary antibody (WO2024164074). This antibody potently blocked VHH-CAR-T activation and cytolytic killing of target cells. When immobilized, anti-VHH antibodies induced strong activation and expansion of VHH CAR-T cells (McComb et al. 2025).

Product Class:	Purified
Clone ID:	2A3
Synonyms:	VHH; Nb; sdAbs; sdAb; Nanobody; Single-domain antibodies; F233-2A3
Amount:	100 μg
Application Codes Clone:	ELISA; IHC; labelling; purification
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Specificity Statement:	This antibody binds distinct epitope in the conserved framework regions of llama-derived VHHs and is reactive with 98% of VHH proteins regardless of their antigenic specificity, with no reactivity to human or mouse IgG and reduced reactivity to conventional llama or alpaca IgG. This antibody fails to react with other isotypes and subtypes of human immunoglobulins like human lgG1 , lgG2, lgG3, lgG4, IgA, slgA and IgM.
Application Notes:	The binding reactivity of this antibody to various sdAbs was determined using ELISA. The binding of this antibody to the VHH did not disrupt VHH/antigen interaction, and so it is appropriate for secondary labeling to assess cellular or tissue reactivity of VHH molecules. The binding ability of this antibody to detect surface expressed sdAb-based CAR constructs was also tested. This antibody could detect Jurkat T cells stably expressing sdCD22 CAR constructs. It was reported that a 1 :1 mix of antibodies 2A3 and 3H12 provides the best overall breadth of binding to diverse VHH proteins. This antibody was also used for the labelling and staining of frozen human tissues using immunohistochemistry. The tissues were first stained with CD22 specific nanobody and the anti-VHH antibody 2A3 was used a secondary antibody (WO2024164074). This antibody potently blocked VHH-CAR-T activation and cytolytic killing of target cells. When immobilized, anti-VHH antibodies induced strong activation and expansion of VHH CAR-T cells (McComb et al. 2025).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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