Vector Labs bAb0895 Anti-CD20 [18B12], Mouse IgG2a mCD28 KIH bispecific, Fc Silent™, kappa (50 μg) Centrifuge Bundle Savings at Sycamore Life Sciences

Description

Product Class: Purified
Clone ID: 18B12
Heavy Chain Modification: Fc Silent™
Synonyms: Ly-44; B-cell differentiation antigen Ly-44; B-lymphocyte antigen CD20; CD20; CD20 antigen; Lymphocyte antigen 44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 2
Amount: 50 μg
Application Codes Clone: FC; IF; WB; depleting; functional assays
Shipping Temperature: Wet Ice
Buffer Composition: PBS with 0.02% Proclin 300.
Original Format: IgG1
Storage Temperature: Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement: This is a bispecific, knob-into-hole heterodimerized antibody, with one arm directed against mouse CD20 (Fab) and one arm directed against mouse CD28 (scFv).
Specificity Statement: This antibody is specific for murine CD20, which is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells. Low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells. Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment.
Application Notes: In the original publication, this antibody was used in FACS and in vivo functional assays to examine how B cell depletion affects autoimmunity (Ahuja et al., 2007). In other studies, this antibody has been used mainly in FACS, immunofluorescence, and various in vitro and in vivo functional experiments. More recent works employing this antibody have shown, for instance, that enhanced phagocytosis of circulating B cells by Kupffer cells represents an important in vivo mechanism for improved activity of glycoengineered anti-CD20 mAbs (Grandjean et al., 2016), that B lymphocytes limit senescence‐driven fibrosis resolution and favor hepatocarcinogenesis in mouse liver injury (Faggioli et al., 2017), and that B cells inhibit the antitumor immunity against an established murine fibrosarcoma (Maglioco et al., 2017).

Product Class:	Purified
Clone ID:	18B12
Heavy Chain Modification:	Fc Silent™
Synonyms:	Ly-44; B-cell differentiation antigen Ly-44; B-lymphocyte antigen CD20; CD20; CD20 antigen; Lymphocyte antigen 44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 2
Amount:	50 μg
Application Codes Clone:	FC; IF; WB; depleting; functional assays
Shipping Temperature:	Wet Ice
Buffer Composition:	PBS with 0.02% Proclin 300.
Original Format:	IgG1
Storage Temperature:	Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
Chimeric Use Statement:	This is a bispecific, knob-into-hole heterodimerized antibody, with one arm directed against mouse CD20 (Fab) and one arm directed against mouse CD28 (scFv).
Specificity Statement:	This antibody is specific for murine CD20, which is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells. Low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells. Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment.
Application Notes:	In the original publication, this antibody was used in FACS and in vivo functional assays to examine how B cell depletion affects autoimmunity (Ahuja et al., 2007). In other studies, this antibody has been used mainly in FACS, immunofluorescence, and various in vitro and in vivo functional experiments. More recent works employing this antibody have shown, for instance, that enhanced phagocytosis of circulating B cells by Kupffer cells represents an important in vivo mechanism for improved activity of glycoengineered anti-CD20 mAbs (Grandjean et al., 2016), that B lymphocytes limit senescence‐driven fibrosis resolution and favor hepatocarcinogenesis in mouse liver injury (Faggioli et al., 2017), and that B cells inhibit the antitumor immunity against an established murine fibrosarcoma (Maglioco et al., 2017).

Specifications

More Information
Brand	Vector Labs
UNSPSC	12161500

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